摘要
目的观察配对盒基因8抗原(paired box gene 8,Pax-8)在腹膜恶性间皮瘤(malignant peritonealmesothelioma,MPM)、高分化乳头状间皮瘤(well-differentiated papillary mesothelioma,WDPM)中的表达,探讨Pax-8在MPM、WDPM与卵巢浆液性肿瘤(ovarian serous tumors,OST)鉴别中的诊断价值。方法收集MPM病例32例,WDPM 12例,良性多囊性间皮瘤6,腺瘤样瘤15例,反应性间皮12例,通过免疫组织化学方法检测这些病变中Pax-8的表达,并分析临床病理学特点。结果5例MPM(15.6%,5/32)表达Pax-8,3例弥漫性中-强阳性,2例局灶性弱-中度阳性;7例WDPM(58.3%,7/12)表达Pax-8,5例弥漫性中-强阳性,2例局灶性弱-中度阳性;1例良性多囊性间皮瘤(16.7%,1/6)Pax-8表达呈局灶性弱-中度阳性;15例腺瘤样瘤均不表达Pax-8;6例反应性间皮(50%,6/12)表达Pax-8,3例弥漫性中-强阳性,3例局灶性弱-中度阳性。结论Pax-8对MPM、WDPM与OST鉴别诊断的价值有限,尤其对于WDPM与交界性或低级别浆液性肿瘤的鉴别不可靠,需要多个标记联合使用,以降低误诊的风险。
Objective To explore the expression of paired box gene 8(Pax-8)in malignant peritoneal mesothelioma(MPM)and well-differentiated papillary mesothelioma(WDPM)and its value in distinguishing MPM,WDPM from ovarian serous tumors(OST).Methods Immunohistochemistry staining was used to assess the expression of Pax-8 in 32 MPM,12 WDPM,6 benign multicystic mesothelioma,15 adenomatous tumor,and 12 reactive mesothelium specimens,and the clinical-pathological characteristics were analyzed Results Pax-8 was expressed in 5 cases(15.6%,5/32)of MPM,in which 3 showed diffusely moderate to strong positive staining and 2 focally weak to moderate positive staining.Pax-8 was expressed in 7 cases(58.3%,7/12)of WDPM,with 5 showing diffusely moderate to strong positive staining and 2 focally weak to moderate positive staining.One case(16.7%,1/6)of benign multicystic mesothelioma was focally weak to moderate positive for Pax-8.All 15 adenomatous tumors were Pax-8 negative.Six cases(50%,6/12)of reactive mesothelium expressed Pax-8,with 3 showing diffusely moderate to strong positive staining and 3 focally weak to moderate positive staining.Conclusion The value of Pax-8 expression in distinguishing MPM,WDPM from OST is limited,especially in distinguishing WDPM from borderline or low-grade serous tumors.Multiple markers need to be applied in combination to reduce the risk of misdiagnosis.
作者
李杜娟
康红
董新敏
黄珊珊
连立飞
Li Dujuan;Kang Hong;Dong Xinmin;Huang Shanshan;Lian Lifei(Department of Pathology,Henan Provincial People’s Hospital,Zhengzhou 450003;Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2021年第3期265-270,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
河南省医学科技攻关计划联合共建项目(2018020401)。
关键词
恶性间皮瘤
腹膜
Pax-8
高分化乳头状间皮瘤
免疫组织化学
Malignant mesothelioma
peritoneum
Pax-8
well-differentiated papillary mesothelioma
immunohistochemistry
