摘要
目的建立快速检测血样中氟马西尼、咪达唑仑、罗哌卡因和地佐辛浓度的方法。方法血样经乙腈沉淀蛋白后,用HPLC-MS/MS检测,在ESI正离子模式下结合多离子反应检测模式,对待测血样进行分析测定。结果待测药物在一定线性范围内,线性关系良好,回收率在95.6%~109.8%,日内精密度在1.06%~4.48%,日间精密度在2.23%~7.48%,氟马西尼最低检出限为0.5 ng/ml,定量限为2 ng/ml;罗哌卡因检出限为0.3 ng/ml,定量限为1 ng/ml;地佐辛检出限为1 ng/ml,定量限为3 ng/ml;咪达唑仑为0.5 ng/ml,定量限为2 ng/ml。结论本文建立了快速检测血中4种麻醉药物的方法,为临床血药浓度监测和司法鉴定提供了依据。
Objective To establish a rapid method for the determination of flumazenic,midazolam,ropivacaine and dezocine in blood samples.Methods Blood samples were treated with acetonitrile protein precipitation,then detected by HPLC-MS/MS under ESI positive ion mode combined with multi-ion reaction detection mode.Results Good linearity was obtained for the four anesthetic drugs,with the recovery ranging from 95.6%to 109.8%,the intra-day precision ranging from 1.06%to 4.48%,and the inter-day precision ranging from 2.23%to 7.48%.The limit of detection for flumazenil was 0.5 ng/ml,and the limit of quantitative was 2 ng/ml.The limit of detection for ropivacaine was 0.3 ng/ml,and the limit of quantitative was 1 ng/ml.The limit of detection for dezocine was 1 ng/ml,and the limit of quantitative was 3 ng/ml.The limit of detection for midazolam was 0.5 ng/ml,and the limit of quantitative was 2 ng/ml.Conclusion The rapid detection method for the four anesthetic drugs in blood was established,which can be applied in drug monitoring and forensic science.
作者
高利娜
夏玮
袁慧雅
徐恩宇
刘俊亭
GAO Li-na;XIA Wei;YUAN Hui-ya;XU En-yu;LIU Jun-ting(School of Forensic Medicine,China Medical University,Shenyang,Liaoning 110014,China)
出处
《中国卫生检验杂志》
CAS
2021年第17期2049-2052,共4页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金项目(81601644
81671864)。
关键词
法医毒物分析
氟马西尼
咪达唑仑
罗哌卡因
地佐辛
液相色谱串联质谱仪
Forensic toxicological analysis
Flumazenil
Dezocine
Ropivacaine
Midazolam
Liquid chromatography tandem mass spectrometer
