摘要
冠状病毒进入细胞后以基因组RNA作为转录本,翻译产生多聚蛋白,多聚蛋白水解后产生16种功能各异的非结构蛋白(Nonstructural Protein,NSP)。其中,NSP7和NSP8对病毒的RNA复制过程和RNA聚合酶活性非常重要。对新型冠状病毒(Severe Acute Respiratory Syndrome Coronavirus 2,SARS⁃CoV⁃2)NSP7和NSP8的保守性分析有助于揭示其生物学特征。SARS⁃CoV⁃2的nsp7和nsp8序列在冠状病毒家族中高度保守。本文综述了NSP7和NSP8的基因功能和蛋白构象。根据相互作用蛋白筛选出氯喹、阿奇霉素等可能的治疗药物。这有助于新型冠状病毒的致病机理研究,相关治疗方法的开发。
After the coronavirus enters the cell,the genomic RNA is used as the transcript,and the translation produces polyprotein.After the polyprotein is hydrolyzed,16 kinds of nonstructural proteins(NSP)with different functions are produced.Among them,NSP7 and NSP8 are very important to the virus RNA replication process and the RNA polymerase activity.Conservation analysis of the new coronavirus(Severe Acute Respiratory Syndrome Coronavirus 2,SARS⁃CoV⁃2)NSP7 and NSP8 will help reveal its biological characteristics.The nsp7 and nsp8 sequences of SARS⁃CoV⁃2 are highly conserved in the coronavirus family.This paper reviews the gene function and protein conformation of NSP7 and NSP8.According to the interacting proteins,possible therapeutic drugs such as chloroquine and azithromycin were screened out.The results are helpful for the study of the pathogenic mechanism of the new coronavirus and the development of related treatment methods.
作者
海洋
谢建平
HAI Yang;XIE Jianping(Institute of Modern Biopharmaceuticals,School of Life Sciences,Southwest University,Chongqing 400715,China)
出处
《生物信息学》
2021年第3期149-158,共10页
Chinese Journal of Bioinformatics
