摘要
目的探究1800 MHz射频电磁场对大鼠的体重和海马体胶原纤维酸性蛋白(GFAP)、神经细胞黏附分子(NCBA)和γ-氨基丁酸(GABA)受体表达的影响。方法购买SD大鼠14只,分为暴露组和对照组,暴露组雌雄比例3∶4,对照组雌雄比例4∶3。暴露频率1800 MHz,强度0.5 mW/cm2。全身暴露3周,每天12 h(20:00~08:00)。暴露结束后,对2组老鼠分别称重。应用免疫组织化学技术对大鼠海马组织切片GFAP、NCBA、GABA受体进行染色,并测定GFAP、NCBA和GABA受体在海马CA1、CA3和DG区的表达变化。结果暴露组与对照组体重指标无明显不同(P>0.05);暴露组海马CA1区、DG区GFAP的表达下降(P<0.05),其他各组比较无明显不同(P>0.05)。结论在1800 MHz,0.5 mW/cm2射频电磁场使SD大鼠暴露3周,大鼠海马CA1和DG区GFAP表达下降,可能对某些脑部疾病有所影响。
Objective To explore the effects of 1800 MHz radio frequency electromagnetic fields on the body weight and expression of Glial fibrillary acidic protein(GFAP),Neural Cell Adhesion Molecule(NCAM)and Gamma-Amino Butyric Acid(GABA)receptors in hippocampus of rats.Methods 14 SD rats were divided into the exposure group and the control group and the ratio of male to female was 3∶4 in the exposure group and 4∶3 in the control group.Exposure frequency 1800 MHz,intensity of 0.5 mW/cm2 RF field.Systemic exposure to three weeks,12 hours a day(20:00-08:00).After the exposure,the rats in the two groups were weighed respectively.Immunohistochemical techniques hippocampus GFAP stained sections,and GFAP NCAM and GABA receptors expression was measured in the hippocampus CA1,CA3 and DG(dentate gyrus)regions.Results The exposure group and the control group showed no significant difference in the body weight(P>0.05);the expression of GFAP in the CA1 and DG areas of the hippocampus decreased(P<0.05),and there was no statistical significance in the other groups(P>0.05).Conclusion At a frequency of 1800 MHz,an intensity of 0.5 mW/cm2 RF electromagnetic field SD rats are exposed for 3 weeks in rat hippocampal CA1 region and DG GFAP expression decreased,which may affect certain brain diseases.
作者
张盛庆宇
舒逍
吴锡南
李志强
武慧欣
张媛
蒋玉融
杨思佳
木云珍
ZHANG Sheng-qingyu;SHU Xiao;WU Xi-nan;LI Zhi-qiang;WU Hui-xin;ZHANG Yuan;JIANG Yu-rong;YANG Shi-jia;MU Yun-zhen(School of PublIc Health,Kunming Medical University,Kunming Yunnan 650500,China;Graduate Faculty,Kunming Medical University,Kunming Yunnan 650500,China;Biomedical Engineering Research Center,Kunming Medical University,Kunming Yunnan 650500,China;School of Basic Medicine,Kunming Medical University,Kunming Yunnan 650500,China)
出处
《昆明医科大学学报》
CAS
2021年第9期13-19,共7页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81560525)
云南省应用基础研究计划基金资助项目(2013FZ063)。
关键词
射频电磁场
生理发育
海马
Radiofrequency electromagnetic field
Physiological growth
Hippocampus
