摘要
目的探讨细针穿刺细胞块石蜡包埋切片技术联合人骨髓内皮细胞标记物-1(HBME-1)、细胞角蛋白19(CK19)、半乳糖凝集素-3(Galectin-3)、CD56检测在甲状腺结节诊断中的临床意义。方法选取2018年7月至2020年5月于该院进行甲状腺结节细针穿刺病理检查的196例患者为研究对象,根据制片方法不同分为细胞涂片组98例,细胞块石蜡包埋切片组98例。细胞块石蜡包埋切片组中26例进行了CK19、CD56、Galectin-3、HBME-1免疫组织化学染色。以术后病理检查结果为“金标准”,分析细针穿刺标本的不同制片方法在甲状腺结节诊断中的价值。结果制作的细胞块石蜡包埋切片中可清楚观察到细胞排列方式及组织结构,染色鲜艳、定位准确、背景清晰。甲状腺乳头状癌HBME-1阳性率为93.75%,CK19阳性率为87.50%,Galectin-3阳性率为81.25%;滤泡性腺瘤CD56阳性率为100.00%。细胞涂片组的检测准确率为71.43%,低于细胞块石蜡包埋切片组的95.92%,且良性符合率和恶性符合率也均低于细胞块石蜡包埋切片组,差异有统计学意义(P<0.05)。结论甲状腺细针穿刺细胞块石蜡包埋切片技术联合CK19、CD56、Galectin-3、HBME-1检测能提高甲状腺结节病理诊断的准确性。
Objective To investigate the clinical significance of fine-needle aspiration cell block paraffin-embedded section technique combined with the detection of human bone marrow endothelial cell marker-1(HBME-1),cytokeratin 19(CK19),Galectin-3 and CD56 in the diagnosis of thyroid nodules.Methods A total of 196 patients who underwent fine-needle aspiration pathological examination of thyroid nodules in the hospital from July 2018 to May 2020 were selected as the research objects.According to different preparation methods,they were divided into 98 cases in the cell smear group and 98 cases in the cell block paraffin-embedded section group.Twenty-six cases in the cell block paraffin-embedded section group were stained by immunohistochemistry for CK19,CD56,Galectin-3 and HBME-1.Took the results of postoperative pathological examination as the"gold standard",the value of different preparation methods of fine-needle aspiration specimens in the diagnosis of thyroid nodules was analyzed.Results The cell arrangement and tissue structure could be clearly observed in the paraffin-embedded section of the cell block,with bright staining,accurate positioning and clear background.The positive rates of HBME-1,CK19 and Galectin-3 in papillary thyroid carcinoma were 93.75%,87.50%and 81.25%respectively.The positive rate of CD56 in follicular adenoma was 100.00%.The detection accuracy of cell smear group was 71.43%,which was lower than 95.92%of cell block paraffin-embedded section group,and the benign coincidence rate and malignant coincidence rate were also lower than those of cell block paraffin-embedded section group,the differences were statistically significant(P<0.05).Conclusion Thyroid fine-needle aspiration cell block paraffin-embedded section technique combined with CK19,CD56,Galectin-3,HBME-1 detection can improve the accuracy of pathological diagnosis of thyroid nodules.
作者
周宁
许婷婷
王帆荣
胡书娅
李韬
ZHOU Ning;XU Tingting;WANG Fanrong;HU Shuya;LI Tao(Department of Pathology,Mianyang 404 Hospital,Mianyang,Sichuan 621000,China)
出处
《检验医学与临床》
CAS
2021年第19期2799-2802,共4页
Laboratory Medicine and Clinic
基金
国家卫生健康委员会疾控局癌症早诊早治项目(GTCZ-2020-SC-51-009)。
