摘要
文章采用乙醇回流提取法提取远志总皂苷,香草醛-冰醋酸显色法测定总皂苷含量,通过单因素试验及正交试验,优选出最佳提取工艺为:20倍量70%乙醇70℃下提取1 h,此条件下总皂苷提取率可达1.900%。本法可有效提高总皂苷得率,为进一步开发和利用提供参考。
Polygala tenuifolia total saponins were extracted by ethanol reflux extraction method,and the content of total saponins was determined by vanillin-glacial acetic acid chromogenic method.Through single factor test and orthogonal test,the optimum extraction process was obtained as follows:extracting 20 times 70%ethanol at 70℃for 1 hour.Under this condition,the extraction rate of total saponins was 1.900%.The method can effectively improve the yield of total saponins and provide reference for further development and utilization.
作者
孙云朋
韩凤波
Sun Yunpeng;Han Fengbo(College of Traditional Chinese Medicine,Jilin University of Agricultural Science and Technology,Jilin 132109,China)
出处
《江苏科技信息》
2021年第31期32-34,共3页
Jiangsu Science and Technology Information
基金
吉林农业科技学院青年基金项目,项目名称:东北远志多糖的分离纯化及其对小鼠学习记忆力的影响,项目编号:吉农院合字第〔20200006〕号。
关键词
远志总皂苷
单因素试验
正交法
Polygala tenuifolia total saponins
single factor test
orthogonal method
