摘要
本研究通过设计单因素试验对发酵条件进行优化,以期提高目标菌株的1,8-桉叶油素产量。结果表明,1,8-桉叶油素内生细菌最佳发酵的工艺条件为:发酵温度为31℃、发酵转速(溶氧)为150 r·min^(−1)、发酵pH值为6.5、发酵时间为20 h。产量由未优化时的2.30 mg·L^(−1)增长至9.68 mg·L^(−1),增长率为320.8%,具有良好的应用前景。
In this paper,the single factor experiment was designed to optimize the fermentation conditions in order to improve the 1,8-cineole yield of the target strain.The results showed that the optimum fermentation conditions were as follows:fermentation temperature was 31℃,fermentation speed(dissolved oxygen)was 150 r·min^(−1),fermentation pH was 6.5 and fermentation time was 20 h.The yield increased from 2.30 mg·L^(−1)(without optimization)to 9.68 mg·L^(−1),with an increase rate of 320.8%,which had a good application prospect.
作者
王鑫
魏琴
莫开林
WANG Xin;WEI Qin;MO Kailin(Yibin University,Yibin 644000,China;Sichuan Academy of Forestry,Chengdu 610081,China)
出处
《四川林业科技》
2021年第5期69-72,共4页
Journal of Sichuan Forestry Science and Technology
基金
四川省科技基础条件平台项目(2018TJPT0035)
四川省科技计划项目(2019YFN0029,2019YFSY0002)。
关键词
油樟
植物内生细菌
1
8-桉叶油素
发酵条件
Cinnamomum longipaniculatum
Endophytic bacteria
1,8-cineole
Fermentation condition
