摘要
目的探讨丙型肝炎病毒(HCV)感染患者血清miR-199及miR-483的表达,分析其对肝癌的诊断价值。方法选取121例HCV感染患者和健康体检正常者50例作为研究对象,其中HCV感染患者分为慢性丙型肝炎(CHC)50例、肝硬化43例和肝癌28例。检测各组血清miR-199及miR-483表达水平和HCV-RNA含量。应用ROC曲线分析miR-199及miR-483对肝癌的诊断价值。Pearson相关分析miR-199与miR-483在HCV感染患者中的相关性。结果肝癌组、肝硬化组和CHC组血清miR-199(3.40±1.25,1.83±0.72,1.70±0.65 vs 0.80±0.14)及miR-483(3.86±1.62,2.04±0.91,1.93±0.82 vs 0.71±0.08)表达水平均明显高于对照组,差异有统计学意义(t=11.904~16.825,均P<0.001)。肝癌组血清miR-199及miR-483表达水平均明显高于CHC组和肝硬化组,差异均有统计学意义(t=11.604~14.817,均P<0.001)。ROC曲线显示,miR-199及miR-483二者联合诊断肝癌的曲线下面积(0.922,95%CI:0.858~0.977)最大,其敏感度和特异度为93.7%和86.4%。相关性分析显示,肝癌患者血清miR-199表达水平与miR-483呈正相关(r=0.803,P<0.001)。结论血清miR-199及miR-483水平在HCV感染患者中明显升高,二者联合对诊断HCV感染导致的肝癌具有较好的诊断价值。
Objective To investigate the expression of serum miR-199 and miR-483 in patients with hepatitis C virus(HCV)infection and analyze their diagnostic value for liver cancer.Methods The 121 patients with HCV infection and 50 healthy people were selected as the research objects.The patients with HCV infection were divided into 50 cases of chronic hepatitis C(CHC),43 cases of liver cirrhosis and 28 cases of liver cancer.The expression levels of serum miR-199 and miR-483 and the content of HCV-RNA in each group were detected.ROC curve was used to analyze of miR-199 and miR-483 in the diagnosis of liver cancer.Pearson correlation analysis were used to analyze the correlation between miR-199 and miR-483 in HCV infected patients.Results The expression levels of miR-199(3.40±1.25,1.83±0.72,1.70±0.65 vs 0.80±0.14)and miR-483(3.86±1.62,2.04±0.91,1.93±0.82 vs 0.71±0.08)in liver cancer group,cirrhosis group and CHC group were significantly higher than those in the control group,the differences were statistically significant(t=11.904~16.825,all P<0.001).The expression levels of miR-199 and miR-483 in liver cancer group were significantly higher than those in CHC group and cirrhosis group,the differences were statistically significant(t=11.604~14.817,all P<0.001).ROC curve showed that the area under curve(0.922,95%CI:0.858~0.977)of the combined diagnosis of liver cancer with miR-199 and miR-483 were the largest,and its sensitivity and specificity were 93.7%and 86.4%,respectively.Correlation analysis showed that the expression level of serum miR-199 in liver cancer group was positively correlated with miR-483(r=0.803,P<0.001).Conclusion The expression levels of serum miR-199 and miR-483 in patients with HCV infection increased significantly,and the combination of the two has a good value in the diagnosis of liver cancer caused by HCV infection.
作者
王楷翔
曹明
陈晖
赵娟
WANG Kai-xiang;CAO Ming;CHEN Hui;ZHAO Juan(Molecular Biology Laboratory,Baoding People’s Hospital,Hebei Baoding 071000,China;Department of Hepatology,Baoding People’s Hospital,Hebei Baoding 071000,China;Department of Clinical Laboratory,the Second Central Hospital of Baoding,Hebei Zhuozhou 072750,China)
出处
《现代检验医学杂志》
CAS
2021年第5期58-61,共4页
Journal of Modern Laboratory Medicine
基金
保定市科学计划与发展指导计划项目(18ZF043)。
