摘要
目的建立高效液相色谱(HPLC)同时测定痔瘘洗液中大黄素和大黄酚含量的方法,为该制剂的质量评价提供依据。方法采用HPLC法同时测定痔瘘洗液中大黄素和大黄酚的含量,色谱柱:Eclipse XDB-C18(4.6 mm×250 mm,5μm);流动相:甲醇-0.1%磷酸水溶液(体积比为80∶20);流速为0.8 ml/min,检测波长为254 nm,柱温为25℃;进样量10μl。结果大黄素、大黄酚的进样量分别在1.90~6.00μg/ml(n=5,R=0.999)和4.00~18.51μg/ml(n=5,R=0.999)范围内与各自的峰面积呈良好的线性关系;平均回收率分别为98.80%、102.50%,相对标准偏差(RSD)分别为0.19%(n=6)、0.02%(n=6)。结论该法具有操作简单、准确、回收率好、精密度和重复性高、分析快速等优点,可作为痔瘘洗液的质量控制方法。
Objective To establish a method for the simultaneous determination of emodin and chrysophanol in Hemorrhoids Fistula Lotion by high-performance liquid chromatography(HPLC)and to provide the basis for the quality evaluation of the preparation.Methods The contents of emodin and chrysophanol in Hemorrhoids Fistula Lotion were simultaneously determined by HPLC.A chromatographic column of Eclipse XDB-C18(4.6 mm×250 mm,5μm)was used.Methanol-0.1%phosphoric acid aqueous solution(80∶20,V/V)was used as mobile phase at a flow rate of 0.8 ml/min,and the detection wavelength was set at 254 nm.The column temperature was 25℃and the injection volume was 10μl.Results There was a good linear relationship between emodin injection volume and its peak area in a range of 1.90~6.00μg/ml(n=5,R=0.999),and there was a good linear relationship between chrysophanol injection volume and its peak area in a range of 4.00~18.51μg/ml(n=5,R=0.999),with the average recovery rates of emodin and chrysophanol being 98.80%and 102.50%,respectively,and the relative standard deviations(RSD)of emodin and chrysophanol being 0.19%(n=6)and 0.02%(n=6),respectively.Conclusion The method has the advantages of simple operation,accuracy,good recovery rate,high precision and good repeatability,and rapid analysis.It can be used for the quality control method of Hemorrhoids Fistula Lotion.
作者
蒙丽斌
潘洪平
MENG Li-bin;PAN Hong-ping(Department of Pharmacy,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China)
出处
《中国临床新医学》
2021年第9期920-924,共5页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
关键词
高效液相色谱
痔瘘洗液
大黄素
大黄酚
同时测定
High-performance liquid chromatography(HPLC)
Hemorrhoids Fistula Lotion
Emodin
Chrysophanol
Simultaneous determination
