摘要
The MADS-box gene plays an important role in regulating plant growth and development.In this study,a SEP3-like MADS-box gene TaSEP3-1 was isolated from bread wheat.The expression patterns of the three homoeologs TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1 were similar,and higher expression levels were detected in floral organs and developing kernels.TaSEP3-D1 was located in the nucleus and cytoplasm and possessed transactivation activity in yeast.Homoeolog sequence polymorphism analysis identified four,three,and four haplotypes of TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1,respectively,and the haplotypes of TaSEP3-D1 had larger effects on agronomic traits than those of TaSEP3-A1 and TaSEP3-B1.D1_h4,significantly associated with heading date,plant height,and other yield-related traits,was the favored haplotype of TaSEP3-D1.Transgenic wheat genotypes overexpressing TaSEP3-D1 exhibited delayed heading and reduced plant height,indicating a role in regulating heading date and plant development.These results shed light on the role of TaSEP3-D1 in wheat plant development.The favored haplotype of TaSEP3-D1 can be applied in breeding to improve plant architecture and yield in wheat.
基金
funded by the Basic Scientific Research Project of Chinese Academy of Agricultural Sciences (Y2019XK10)
the National Key Research and Development Program of China (2016YFD0100102, 2016YFD0100302)。
