摘要
目的探讨白血病患儿的免疫复合物与疾病特征及BCG免疫治疗后患者抗体反应的相关性。方法选取60例白血病患儿作为研究对象,经诊断有41例患儿为急性淋巴细胞白血病,19例为T细胞白血病。另取同期73例无血液疾病的患儿作为对照组。对所有患者应用C1q(抗补体1q抗体)检测法联合聚乙二醇沉淀法进行诊断,对比2组患儿血清中免疫复合物发生率,分析白血病患儿的免疫复合物与疾病特征及BCG免疫治疗后患者抗体反应的相关性。结果41例急性淋巴细胞白血病患儿血清平均结合125 I-C1q为(12.46±1.64)%,19例T细胞白血病患儿血清中125 I-C1q的的结合率为(17.47±2.69)%,而73例对照组患儿血清平均结合125 I-C1q为(6.52±1.25)%,且该组患儿中没有1例高于15%(超过15%的125 I-C1q结合被认为是阳性),3组间差异有显著性(P<0.05)。在PEG沉淀试验中,41例急性淋巴细胞白血病患儿的诊断血清的外径读数平均为0.273±0.097,高于对照组的血清平均数0.188±0.054(P<0.001)。C1q与PEG结果之间有很强的相关性(P<0.001)。13例诊断血清结合率超过15%的患儿中有3例来自41例急性淋巴细胞白血病患儿,另10例来自19例T细胞白血病患儿。这13例患儿中有2例在16个月和20个月时出现骨髓复发,其余11例在治疗后8~18个月仍处于缓解状态。初发白细胞计数高但C1q-BA正常的8例患者中有2例复发。结论免疫复合物在不同类型白血病中的发生存在差异,而在ALL患儿中,其可能与进行性疾病或预后不良无关。
Objective To investigate the relationship between immune complex and disease characteristics in children with leukemia and antibody reaction after BCG immunotherapy.Methods 60 children with leukemia were selected as the research objects.After diagnosis,41 children were diagnosed as acute lymphoblastic leukemia and 19 were T-cell leukemia.and 73 cases of children without blood disease were selected as the control group.All patients were diagnosed by C1q(anti complement 1q antibody)detection method combined with polyethylene glycol precipitation method.The incidence of immune complexes in the sera of the 2 groups of children was compared,and the correlation between immune complexes and disease characteristics in leukemia children and the patient's antibody response after BCG immunotherapy was analyzed.Results The average combined 125 I-C1q in serum of 41 children with acute lymphoblastic leukemia was(12.46±1.64)%,the binding rate of 125 I-C1q in the serum of 19 children with T-cell leukemia was(17.47±2.69)%.And 73 children in the control group had an average 125 I-C1q binding of(6.52±1.25)%,and none of the children in this group was higher than 15%(more than 15%of 125 I-C1q binding was considered positive).The difference among the 3 groups was significant(P<0.05).In the PEG precipitation test,the average OD reading of the diagnostic serum of 41 children with acute lymphoblastic leukemia was 0.273±0.097,which was higher than the average serum value of 0.188±0.054 in the control group(P<0.001),there was a strong correlation between C1q and PEG results(P<0.001).Of the 13 children with a diagnostic serum conjugation rate of more than 15%,3 were from 41 children with acute lymphoblastic leukemia,and the other 10 were from 19 children with T-cell leukemia.Bone marrow recurrence occurred in 2 of the 13 patients at 16 and 20 months.The remaining 11 cases were still in remission 8-18 months after treatment.Two of the 8 patients with high initial WBC count but normal C1q Ba recurred.Conclusion There is a difference in t
作者
赵惠娅
高慧
郭雯
ZHAO Huiya;GAO Hui;GUO Wen(Children's Hospital of Kaifeng,Kaifeng,475000)
出处
《实用癌症杂志》
2021年第9期1524-1527,共4页
The Practical Journal of Cancer
基金
国家科技重大专项“十三五”课题(编号:2017ZX10305501004)。
