摘要
目的探讨启动子甲基化调控的抑瘤素M受体(OSMR)在胃癌中的表达及其作用。方法在UCSC Xena数据库中采用Studentt检验和皮尔森相关性检验分析OSMR基因及其启动子甲基化在胃癌中的表达及其调控关系。收集武汉大学中南医院2014年2月至2016年6月的80例人胃癌组织,经免疫组织化学实验检测OSMR蛋白表达水平,然后采用卡方检验分析OSMR蛋白表达量与患者临床资料的关系。结果 UCSC Xena数据库分析表明OSMR mRNA在胃癌组织中(14.752±7.593)高于癌旁组织(8.098±3.457,t=-8.938,P<0.01),而OSMR启动子甲基化在胃癌组织中(0.195±0.104)低于癌旁组织(0.463±0.187,t=9.372,P<0.01)。相关分析结果表明OSMR启动子甲基化与其基因表达呈负相关(R=-0.562,t=-14.517,P<0.01)。Kaplan-Meier生存分析结果表明OSMR蛋白表达水平与患者总体生存时间负相关[风险比(HR)=1.535,95%可信区间(CI):1.214~1.873,P<0.01]。χ^(2)检验结果表明OSMR蛋白高表达与肿瘤分化(χ^(2)=9.389,P<0.01)、TNM分期(χ^(2)=8.166,P<0.01)和淋巴结转移(χ^(2)=4.233,P<0.05)明显相关。结论启动子甲基化调控的OSMR在胃癌中高表达,且其表达量与患者的预后呈负相关。
Objective To investigate the expression and significance of promoter methylation regulated oncostatin M receptor(OSMR)in gastric cancer.Methods Chi-square test and pearson correlation analysis were respectivelty used to explore the expression levels and regulation relationship of OSMR mRNA and its promoter methylation in gastric cancer in UCSC Xena database.Immunohistochemistry was used to detect the expression of OSMR protein in 80 cases of gastric cancer tissues.Furthermore,we analyzed the relationship between OSMR protein expression and the clinical features and survival of 80 patients with gastric cancer.Results Bioinformatics analysis through UCSC Xena database suggested that OSMR mRNA expression was elevated(14.752±7.593 vs.8.098±3.457,t=-8.938,P<0.01),whereas the promoter methylation level of OSMR was reduced(0.195±0.104 vs.0.463±0.187,t=9.372,P<0.01)in gastric cancer.Furthermore,correlation analysis revealed that OSMR mRNA level was negatively related with its promoter methylation(R=-0.562,t=-14.517,P<0.01)in gastric cancer.Kaplan-Meier survival analysis suggested that OSMR protein expression was significantly associated with poor 5-year overall survival[hazard ratio(HR)=1.535,95%confidence interval(CI):1.214-1.873,P<0.01].In addition,the high expression of OSMR protein was closely related to tumor differentiation degree(χ^(2)=9.389,P<0.01),TNM stage(χ^(2)=8.166,P<0.01)and lymphatic metastasis(χ^(2)=4.233,P<0.05).Conclusion Promoter methylation regulated OSMR expression is increased and asscciated with poor prognosis in gastric cancer.
作者
卫海燕
窦婷亭
王国洲
刘训碧
李霞
方效昌
郑新宇
陈天亮
李炫飞
Wei Haiyan;Dou Tingting;Wang Guozhou;Liu Xunbi;Li Xia;Fang Xiaochang;Zheng Xinyu;Chen Tianliang;Li Xuanfei(Department of Public Health,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China;Department of Gastrointestinal Surgery,Zhongnan Hospital,Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Clinical Cancer Study Center of Hubei Provence,Key Laboratory of Tumor Biological Behavior of Hubei Provence,Wuhan 430071,China;Department of Breast Cancer Surgery,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第9期1772-1774,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(81770283、82070302、81902018)
武汉市腹膜癌临床医学研究中心资助项目(2015060911020462)
湖北省自然科学基金青年项目(2019CFB109)
武汉大学中南医院创新培育基金(znpy2018004)。
关键词
启动子甲基化
胃癌
预后
Promoter methylation
Gastric cancer
Prognosis
