摘要
为了探究不同构型烟碱在人体内与生物蛋白的相互作用,利用多种荧光光谱手段,研究了S型和R型烟碱与胃膜素(GIh)的相互作用机理和结合模式,以及烟碱对GIh结构的影响。结果表明:①两种构型烟碱与GIh之间均存在由疏水作用力占主导的较弱的相互作用,且S型烟碱与GIh间的结合强度略强于R型。②烟碱两种异构体均对温度比较敏感,一定程度提高温度有利于烟碱与GIh间的结合。③S型和R型烟碱对GIh的荧光淬灭机理相同,均为静态淬灭方式。两种构型烟碱作用于GIh时均会引起氨基酸残基周围微环境发生一定程度的改变。CD结果显示,烟碱在与GIh结合时具有构型翻转的现象,表明烟碱与GIh的结合可能是由构型翻转诱导的。
To explore the interaction between nicotine(NIC)of different configurations and biological proteins in human bodies,a variety of spectroscopic techniques were used to study the interaction mechanism and binding mode between the two isomers(S-type and R-type)of NIC and gastrin I Human(GIh)and the effect of NIC on GIh structure.The results showed that:1)There were weaker bindings between the two isomers of NIC and GIh,in which hydrophobic interaction was the dominated one.The binding strength between S-NIC and GIh was slightly stronger than R-NIC.2)Both isomers of NIC were sensitive to temperature.Raising temperature within a certain extent was beneficial to the binding between NIC and GIh.3)The fluorescence quenching mechanisms of S-and R-NIC for GIh were the same,both were static quenching modes.The microenvironment around amino acid residues was changed to a certain extent when the two nicotine configurations acted on GIH.Based on circular dichroism(CD)results,the configuration inversion of NIC occurred while binding to GIh,which indicated that the binding between NIC and GIh might be induced by the inversion of NIC configuration.
作者
杨继
朱瑞芝
向能军
司晓喜
何沛
刘春波
唐石云
张凤梅
王强
刘志华
蔡炳彪
YANG Ji;ZHU Ruizhi;XIANG Nengjun;SI Xiaoxi;HE Pei;LIU Chunbo;TANG Shiyun;ZHANG Fengmei;WANG Qiang;LIU Zhihua;CAI Bingbiao(Technology Center of China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China;Process Quality Department of Hongta Tobacco(Group)Co.,Ltd.,Yuxi 653100,Yunnan,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2021年第9期48-57,共10页
Tobacco Science & Technology
基金
国家烟草专卖局重大专项“新型烟草产品中烟碱/烟碱盐释放机理及感官作用机制研究”[110202001007(XX-03)]
国家烟草专卖局重大专项“加热不燃烧卷烟的气溶胶理化特性表征及调控技术研究”[110201901002(XX-02)]
云南中烟工业有限责任公司科技开发计划“新型烟草产品关键技术开发”(2019XY02)
云南中烟工业有限责任公司科技开发计划项目“加热卷烟添加剂安全性评价技术研究及数字化平台构建”(2021JC05)。
关键词
烟碱
构型
胃膜素
作用机理
分子光谱法
构型翻转
Nicotine
Configuration
Gastrin I Human
Interaction mechanism
Molecular spectroscopy
Configuration inversion
