摘要
通过测定转匍匐翦股颖小热激蛋白基因AsHSP26.8a拟南芥株系的根长、植株质量、蒸腾速率、气孔导度、净光合速率以及PSⅡ光能转化效率,探讨AsHSP26.8a对拟南芥光合作用的影响,并进一步通过qRT-PCR分析了拟南芥内源光合相关基因(rbcL、psaA、psbA、psaE、clpP、rpoB、rpo A、accD)的表达情况来揭示AsHSP26.8a参与光合作用的调控途径。结果表明:与野生型相比,转基因拟南芥根长显著缩短,鲜质量和干质量下降;光合速率、蒸腾速率、气孔导度和PSⅡ光能转化效率均有所下降,光合相关基因的表达也有所下调。由此认为AsHSP26.8a抑制了转基因拟南芥光合相关基因的表达,使转基因拟南芥的光合作用减弱,从而进一步抑制了转基因拟南芥根系生长和干物质的积累。
In this paper,effects of a creeping bentgrass small heat shock protein,As HSP26.8 a,on photosynthesis in transgenic Arabidopsis thaliana were studied by measuring root length,plant weight,transpiration rate,stomatal conductance,net photosynthetic rate and PSⅡlight energy conversion efficiency of As HSP26.8 transgenic A.thaliana.Furthermore,the expression of endogenous photosynthesis-related genes(rbc L,psa A,psb A,psa E,clp P,rpo B,rpo A,acc D)in A.thaliana were analyzed by qRT-PCR to reveal the regulation pathway of As HSP26.8 a in plant photosynthesis.Results showed that compared with the wild type,the root length of transgenic A.thaliana was significantly shortened,the fresh weight and dry weight decreased,and the photosynthetic rate,transpiration rate,stomatal conductance and photochemical efficiency of transgenic A.thaliana decreased,and the expression of photosynthesis-related genes in transgenic A.thaliana was also down-regulated.It is suggested that As HSP26.8 a inhibits the expression of photosynthesis-related genes in transgenic A.thaliana,which weakened the photosynthesis of transgenic A.thaliana,and subsequently leaded to the inhibition of root growth and dry matter accumulation of transgenic A.thaliana.
作者
朱俊飞
李鑫
董康挺
唐志菲
边秀举
王丽宏
李会彬
孙鑫博
ZHU Junfei;LI Xin;DONG Kangting;TANG Zhifei;BIAN Xiuju;WANG Lihong;LI Huibin;SUN Xinbo(Key Laboratory of Crop Growth Regulation of Hebei Province,College of Agronomy,Hebei Agricultural University,Baoding,Hebei 071000,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2021年第8期1619-1625,共7页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31701955)
河北省自然科学基金优秀青年项目(C2018204056)
河北省现代农业产业技术体系草业创新团队专项资金项目(HBCT2018160203)。
