摘要
目的:分析人免疫缺陷病毒/艾滋病(HIV/AIDS)患者抗病毒治疗前HIV-1耐药以及影响因素,探讨HIV/AIDS患者外周血CD_(8)^(+)T细胞CD38表达(CD_(8)^(+)CD_(38)^(+)T淋巴细胞百分比)与CD_(4)^(+)T淋巴细胞计数的相关性。方法:选择2016年3月至2019年12月我院接诊的442例HIV/AIDS患者(HIV/AIDS组)和163例同期于我院进行体检的健康志愿者(对照组),HIV/AIDS组扩增pol基因,进行HIV-1基因耐药分析,检测CD_(8)^(+)CD_(38)^(+)T淋巴细胞百分比、CD_(4)^(+)T淋巴细胞计数、CD_(8)^(+)T淋巴细胞计数。分析HIV/AIDS患者HIV-1耐药的影响因素,分析CD_(8)^(+)CD_(38)^(+)T淋巴细胞百分比与CD_(4)^(+)T淋巴细胞计数、CD_(8)^(+)T淋巴细胞计数相关性。结果:HIV/AIDS组442例HIV/AIDS患者中376例获得HIV-1 pol基因序列,HIV-1耐药35例,耐药率9.31%(35/376)。单因素分析结果显示耐药组和非耐药组在年龄、文化程度、感染途径、HIV病毒载量方面差异有统计学意义(P<0.05)。多因素Logistic回归分析结果显示同性性传播、注射吸毒、高HIV病毒载量是HIV/AIDS患者抗病毒治疗前HIV-1耐药的危险因素(P<0.05)。HIV/AIDS组外周血CD_(4)^(+)T淋巴细胞计数、CD_(8)^(+)T淋巴细胞计数低于对照组(P<0.05),CD_(8)^(+)CD_(38)^(+)T淋巴细胞百分比高于对照组(P<0.05)。CD_(8)^(+)CD_(38)^(+)T淋巴细胞百分比与CD_(4)^(+)T淋巴细胞计数、CD_(8)^(+)T淋巴细胞计数呈负相关(P<0.05)。结论:抗病毒治疗前HIV/AIDS患者存在一定HIV-1耐药率,传播途径、HIV-1病毒载量与HIV-1耐药有关。CD_(8)^(+)T细胞表面CD38过表达与HIV/AIDS患者CD_(4)^(+)T T细胞的过度消耗有关。
Objective:To analyze HIV-1 drug resistance and influencing factors before antiviral therapy in human immunodeficiency virus/AIDS(HIV/AIDS)patients,and to investigate the correlation between CD_(8)^(+)T cells CD38 expression(percentage of CD_(8)^(+)CD_(38)^(+)T lymphocytes)and CD_(4)^(+)T lymphocyte count in peripheral blood of HIV/AIDS patients.Methods:442 HIV/AIDS patients(HIV/AIDS group)and 163 healthy volunteers(control group)who were admitted to our hospital from March 2016 to December 2019 were selected.The HIV/AIDS group amplified pol gene,and the drug resistance of HIV-1 gene were analyzed.The percentage of CD_(8)^(+)CD_(38)^(+)T lymphocytes,CD_(4)^(+)T lymphocyte count and CD_(8)^(+)T lymphocyte count were detected.The influencing factors of HIV-1 drug resistance in HIV/AIDS patients were analyzed,and the correlation between the percentage of CD_(8)^(+)CD_(38)^(+)T lymphocytes and CD_(4)^(+)T lymphocyte count and CD_(8)^(+)T lymphocyte count were analyzed.Results:In the HIV/AIDS group,376 cases of the 442 cases of HIV/AIDS patients obtained HIV-1 Pol gene sequence,and 35 cases were HIV-1 drug resistance,with drug resistance rate of 9.31%(35/376).Univariate analysis results showed that the drug-resistant group and non-drug-resistant group had statistically significant differences in age,educational level,route of infection and HIV viral load(P<0.05).Multivariate Logistic regression analysis showed that homosexual sexual transmission,injecting drug abuse and high HIV viral load were the risk factors for HIV-1 drug resistance in HIV/AIDS patients before antiviral treatment(P<0.05).The CD_(4)^(+)T lymphocyte count and CD_(8)^(+)T lymphocyte count in peripheral blood of HIV/AIDS group were lower than those of control group(P<0.05),and the percentage of CD_(8)^(+)CD_(38)^(+)T lymphocytes was higher than that of control group(P<0.05).The percentage of CD_(8)^(+)CD_(38)^(+)T lymphocytes was negatively correlated with CD_(4)^(+)T lymphocyte count and CD_(8)^(+)T lymphocyte count(P<0.05).Conclusion:There is a certa
作者
艾尼瓦尔•吾拉木
帕丽扎提•阿不都热依木
刘晓萱
卢婷
马文婷
Aniwar·wulamu;Palizati•abudureyimu;LIU Xiao-xuan;LU Ting;MA Wen-ting(First Department of Infectious Disease,The eighth Affiliated Hospital ofXinjiang Medical University,Urumqi,Xinjiang,830000,China;Inspection Center,The First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830000,China)
出处
《现代生物医学进展》
CAS
2021年第16期3092-3096,共5页
Progress in Modern Biomedicine
基金
新疆维吾尔自治区自然科学基金项目(2017D01C17)。
