摘要
目的探讨树突状细胞(DC)介导心肌梗死(AMI)大鼠特异性细胞免疫对心室功能改善的作用。方法购自北京医疗器械检验所(动物实验中心)清洁级雄性大鼠24只,构建AMI手术模型后将其随机分成A、B、C三组,每组各8只,A组为假手术大鼠、B组为AMI大鼠、C组为接受DC干预的AMI大鼠。记录大鼠生存情况;每组4只用于DC、血流动力学指标和炎性因子检测,另4只用于HE染色检测细胞炎性浸润、细胞凋亡及心肌梗死情况。结果C组生存率高于A组(P<0.05);AMI后第3 d可检出DC相关标志物,第7 d达高峰,第14 d后下降;C组CD40、CD80、CD86、MHCⅠ、MHCⅡ表达水平高于A、B组(P<0.05);流式分析结果显示,DC可获取CD4^(^(+))CD25^(+)纯度>90%,且可有效诱导Treg细胞群;Treg在大鼠心脏和淋巴结中均有表达,且C组表达水平高于A、B组(P<0.05);C组动力学指标、炎性因子水平优于A、B组(P<0.05);C组心肌梗塞面积、胶原体积分数(CVF)低于A、B组(P<0.05),且基质金属蛋白酶2(MMP-2)活性高于A、B组(P<0.05);C组炎性细胞计数、细胞凋亡<A、B组(P<0.05)。结论DC可激活AMI后特异性细胞免疫,修复炎性细胞,进一步改善心室重塑及功能,提高AMI大鼠生存率。
Objective To investigate the improving effect of specific cellular immunity mediated by dendritic cells(DC)on ventricular function in rats with acute myocardial infarction(AMI).Methods Male rats(n=24)were randomly divided into group A(sham-operation group),group B(AMI group)and group C(AMI^(+)DC group,each n=8)after AMI model established.The rat survival situation was recorded.The changes of DC,hemodynamic indexes and inflammatory factors were detected in 4 rats in each group,and inflammatory infiltration,apoptosis and myocardial infarction were detected by using HE staining in other 4 rats in each group.Results The survival rate was higher in group C than that in group A(P<0.05).DC-related biomarkers were detected on the 3rd d after AMI,reached peak on the 7th d and decreased on the 14th d.The expressions of CD40,CD80,CD86,MHC Ⅰ and MHCⅡwere higher in group C than those in group B and group A(P<0.05).The results of flow cytometry analysis showed that DC could obtain CD4^(+)CD25^(+)with purity greater than 90%,and induce effectively Treg cell population.The expression of Treg could be observed in rat heart and lymph node,which was higher in group C than that in group B and group A(P<0.05).The hemodynamic indexes and inflammatory factors were superior in group C to those in group B and group A(P<0.05).The area of myocardial infarction and collagen volume fraction(CVF)were lower(P<0.05),and activity of matrix metalloproteinase 2(MMP-2)was higher(P<0.05)in group C than those in group B and group A.The count of inflammatory cells and apoptosis rate were lower in group C than those in group B and group A(P<0.05).Conclusion DC can activate specific cellular immunity,repair inflammatory cells,further improve ventricular remodeling and function,and improve survival rate of AMI rats.
作者
王永杰
方小丽
刘仕强
王杰
陈旭
汪华
何文武
Wang Yongjie;Fang Xiaoli;Liu Shiqiang;Wang Jie;Chen Xu;Wang Hua;He Wenwu(Department of Chest and Heart Surgery,Central Hospital of Nanchong City,Sichuan Province,Nanchong 637000,China;不详)
出处
《中国循证心血管医学杂志》
2021年第9期1068-1072,共5页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
四川省科技计划项目(2019JDRC0076)。
关键词
心肌梗死
树突状细胞
特异性细胞免疫
心室功能改善
大鼠
Myocardial infarction
Dendritic cells
Specific cellular immunity
Improvement of ventricular function
Rats
