摘要
应用连续流动−分光光度法测定加热不燃烧卷烟专用新型薄片中甲醛.称取剪碎的薄片样品(0.5 cm×0.5 cm)0.5000 g,加入0.01 mol/L的氢氧化钠溶液50 mL和1.5 g活性炭,振荡1 h,过滤,收集滤液.采用连续流动−分光光度法,薄片中甲醛经0.01 mol/L氢氧化钠溶液萃取后,甲醛的醛基进一步与乙酰丙酮在线反应生成420 nm处有吸收的显色复合物,通过监测420 nm处吸光度的变化,可推算得到薄片中的甲醛含量.结果表明,①该方法仪器流路最佳配制为样品溶液和去离子水均用用红/红泵管,乙酰丙酮用黑/黑泵管,反应混合圈为20匝,流路添加透析器;②在优化的实验条件下,该方法检出限为1.05 mg/kg,定量限为3.50 mg/kg,加标回收率为95.5%~110.0%,相对标准偏差为0.18%~0.71%;③该方法较高效液相色谱法成本低廉、前处理简单、测定快速、定量准确、检出限低,非常适合薄片企业生产质量监管的需要.
Formaldehyde in a new type of reconstituted tobacco for heating non-combustion cigarettes was determinated by continuous flow-spectrophotometer.Taking 0.5000 g of reconstituted tobacco(0.5 cm×0.5 cm)as sample,and 50 mL of 0.01 mol/L sodium hydroxide solution and 1.5 g activated carbon were added,the sample was vibrated for 1 h,filtered and the filtrate was collected.Continuous flow-the spectrophotometer was adopted,sodium formate was formed by the reaction of formaldehyde with sodium hydroxide,and the aldehyde group of sodium formate reacted with acetylacetone on-line to form an absorbable colour complex at 420 nm,the formaldehyde content in the reconstituted tobacco can be calculated.The results show that:①The optimal pump tube configuration for the instrument is to use red/red for sample solution and deionized water and black/black for acetylacetone,the reaction mixing circle is 20 turns,and add a dialyzer to the analysis stream;②The detection limit was 1.05 mg/kg,the quantitative limit was 3.50 mg/kg,the recovery was 95.5%~110.0%and the relative standard deviation was 0.18%~0.71%;③The method has the advantages of low cost,simple pretreatment,rapid determination,accurate quantification and low detection limit as compared with HPLC method,and is very suitable for the need of quality control of thin slice enterprises.
作者
王春琼
陈丹
蔡洁云
张轲
李苓
张燕
顾健龙
孙浩巍
曾天宝
彭丽娟
WANG Chun-qiong;CHEN Dan;CAI Jie-yun;ZHANG Ke;LI Ling;ZHANG Yan;GU Jian-long;SUN Hao-wei;ZENG Tian-bao;PENG Li-juan(Yunnan Tobacco Quality Supervision and Test Station,Kunming 650106,Yunnan,China;Shenzhen E-Zheng Tech Co.,Ltd.,Shenzhen 518000,Guangdong,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第5期996-1003,共8页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省烟草专卖局科技项目(2020530000241036).
关键词
分光光度法
连续流动分析
甲醛
加热不燃烧
新型烟草薄片
spectrophotometer
continuous flow analysis
formaldehyde
non-combustion by heating
new reconstituted tobacco
