摘要
目的探讨DNA甲基化转移酶1(DNMT1)基因多态性位点与乳腺癌临床病理特征的相关性。方法采用SNaPshot技术检测68例乳腺癌患者(病例组)和80例体检健康女性(对照组)外周血中DNMT1基因rs2114724、rs2228611、rs8101866和rs16999593共4个多态性位点的基因分型。结果病例组和对照组DNMT1的4个位点的基因型比较,差异均无统计学意义(P>0.05);病例组各基因型在乳腺癌临床病理学指标间比较,差异均无统计学意义(P>0.05)。DNMT1基因rs2114724、rs2228611、rs81018663个位点的单倍型T、A、T能增加患乳腺癌的风险。结论DNMT1基因rs2114724、rs2228611、rs8101866和rs16999593位点单核苷酸多态性与患乳腺癌的风险有一定的相关性。
Objective To investigate the correlation between DNA methyltransferase 1(DNMT1)gene polymorphism and clinicopathological features of breast cancer.Methods Four polymorphic loci of DNMT1 gene rs2114724,RS2228611,RS8101866 and RS16999593 in peripheral blood of 68 breast cancer patients(case group)and 80 healthy women(control group)were detected by SNaPshot technique.Results There was no significant difference in genotype of 4 DNMT1 loci between the patient group and the control group(P>0.05).There was no significant difference in the clinical pathological indexes of breast cancer genotype in case group(P>0.05).The haplotype T,A,T at rs2114724,RS2228611 and RS8101866 of DNMT1 gene could increase the risk of breast cancer.Conclusion Single nucleotide polymorphisms of rs2114724,rs2228611,rs8101866,and rs16999593 in DNMT1 are associated with the risk of breast cancer.
作者
王蓉
潘小平
赖金国
杜利军
杨园
WANG Rong;PAN Xiaoping;LAI Jinguo;DU Lijun;YANG Yuan(Department of Clinical Laboratory,Huadu District People′s Hospital,Guangzhou,Guangdong 510800,China)
出处
《国际检验医学杂志》
CAS
2021年第18期2222-2227,共6页
International Journal of Laboratory Medicine
基金
广东省广州市花都区科技计划项目(18-HDWS-030)。
关键词
DNA甲基化转移酶1
乳腺癌
单核苷酸多态性
DNA methylation transferase 1
breast cancer
single nucleotide polymorphism
