摘要
目的:探讨维生素D_(3)对实验性自身免疫性甲状腺炎大鼠炎症、辅助性T细胞1/辅助性T细胞2(helper T cell 1/helper T cell 2,Th1/Th2)及单核细胞趋化蛋白1/细胞表面趋化因子受体2(monocyte chemoattractant protein-1/cell surface chemokine receptor 2,MCP-1/CCR2)信号的影响。方法:将Lewis大鼠按随机数字表法分为对照组(n=10)、模型组(n=15)和治疗组(n=15),采用免疫猪甲状腺球蛋白(porcine thyroglobulin,pTG)诱导建立实验性自身免疫性甲状腺炎(experimental autoimmune thyroiditis,EAT)大鼠模型,治疗组按照每只5 mg/kg的剂量腹腔注射1,25-二羟维生素D_(3)[1,25-(OH)2D_(3)],隔天1次,共用4周,其余2组均给予等体积生理盐水。采用苏木素-伊红(hematoxylin-eosin stain,HE)染色观察大鼠甲状腺组织形态学变化;酶联免疫吸附法(enzyme-linked immunosorbentassay,ELISA)检测血清中促甲状腺激素(thyroid stimulating hormone,TSH)、自身抗体抗甲状腺球蛋白抗体(anti-thyroglobulin antibody,TGAb)、甲状腺过氧化物酶抗体(anti-thyroid peroxidase antibody,TPOAb)及细胞因子干扰素-γ(interferon,IFN-γ)、白细胞介素-12(interleukin-12,IL-12)、白细胞介素-4(interleukin-4,IL-4)、白细胞介素-10(interleukin-10,IL-10),逐步线性回归分析TSH与自身抗体、细胞因子的关系;免疫组织化学检测甲状腺组织中的NF-κB p65;Western blot检测甲状腺组织中MCP-1/CCR2信号轴。结果:模型组甲状腺滤泡结构破坏、炎性细胞浸润,治疗组甲状腺病理变化明显改善并趋向于对照组。治疗组TSH、TGAb、TPOAb水平与模型组相比均明显降低(t=4.000、2.603、5.279,P=0.000、0.015、0.000),但仍明显高于对照组(t=2.400、2.216、7.392,P=0.025、0.037、0.000)。与模型组相比,治疗组IL-4、IL-10水平明显升高(t=3.522、2.432,P=0.001、0.022),IFN-γ、IL-12水平及IFN-γ/IL-4、IL-12/IL-10比值明显降低(t=2.940、4.700、5.416、8.178,P=0.007、0.000、0.000、0.000),均趋向于对照组水平。逐�
Objective:To investigate the effect of vitamin D_(3)on inflammation,helper T cell 1/helper T cell 2(Th1/Th2)and monocyte chemoattractant protein-1/cell surface chemokine receptor 2(MCP-1/CCR2)signal in experimental autoimmune thyroiditis(EAT)rats.Methods:Lewis rats were randomly divided into the control group(n=10),the model group(n=15)and the treatment group(n=15).The rat model of EAT was established by immunizing porcine thyroglobulin(p TG).The treatment group received intraperitoneal injection of 1,25-(OH)2 vitamin D_(3)at the dose of 5 mg/kg,once every other day for consecutive four weeks,and the other two groups were given normal saline of equal volume.The morphological changes of thyroid tissue in rats were observed by hematoxylineosin(HE)staining.Serum thyroid stimulating hormone(TSH),anti-thyroglobulin antibody(TGAb),anti-thyroid peroxidase antibody(TPOAb)and cytokines interferon-γ(IFN-γ),interleukin-12(IL-12),interleukin-14(IL-4)and interleukin-10(IL-10)were detected by enzyme-linked immunosorbent assay(ELISA).The relationship between TSH and autoantibodies and cytokines was analyzed by stepwise linear regression.NF-κB p65 in thyroid tissue was detected by immunohistochemistry.MCP-1/CCR2 signal axis in thyroid tissue was analyzed by Western blot.Results:In the model group,thyroid follicles were damaged and inflammatory cells were infiltrated,while the pathological changes of thyroid in the treatment group were significantly improved and tended to the control group.The levels of TSH,TGAb and TPOAb in the treatment group were significantly lower than those in the model group(t=4.000,2.603,5.279;P=0.000,0.015,0.000),but were still significantly higher than those in the control group(t=2.400,2.216,7.392;P=0.025,0.037,0.000).Compared with the model group,the levels of IL-4 and IL-10 were significantly increased in the treatment group(t=3.522,2.432;P=0.001,0.022),and the levels of IFN-γ,IL-12 and IFN-γ/IL-4 and IL-12/IL-10 were significantly decreased in the treatment group(t=2.940,4.700,5.416,8.178;P=0.0
作者
侯丽萍
耿建林
谷巍
刘晴晴
Hou Liping;Geng Jianlin;Gu Wei;Liu Qingqing(Department of Endocrinology,Hallison International Peace Hospital)
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2021年第8期921-926,共6页
Journal of Chongqing Medical University
