摘要
[目的]本试验旨在研究染料木黄酮对产蛋后期种母鸡繁殖性能的调控作用,为开发种鸡用饲粮添加剂提供参考。[方法]试验选用180只体重和生理状态相近的58周龄栖岭草鸡种母鸡,随机分为3组:对照组、低剂量染料木黄酮(L-Gen)组和高剂量染料木黄酮(H-Gen)组。预饲期2周,正式试验期4周。在预饲期,所有组统一饲喂玉米-豆粕型基础日粮;在正式试验期,对照组继续饲喂基础日粮,L-Gen和H-Gen组日粮分别在基础日粮中添加20和40 mg·kg^(-1)染料木黄酮。[结果]饲粮中添加20 mg·kg^(-1)染料木黄酮显著提高试验第3、4周的平均蛋重、蛋壳厚度和下丘脑中GnRH mRNA表达水平(P<0.05);饲粮中添加40 mg·kg^(-1)染料木黄酮显著提高种母鸡第3、4周的产蛋率和第4周平均蛋重(P<0.05),以及血清中雌激素和孕酮的含量(P<0.05);L-Gen和H-Gen组种蛋蛋黄色泽等级和蛋壳强度较对照组均显著提高(P<0.05)。染料木黄酮处理显著提高卵巢组织中SOD活性和T-AOC含量(P<0.05),并上调小黄卵泡中ERα和FSHR mRNA表达水平(P<0.05)。[结论]日粮中添加染料木黄酮能够激活下丘脑-垂体-性腺轴,增强卵巢组织抗氧化以及生殖激素的分泌功能,提高产蛋后期种母鸡的繁殖性能。
[Objectives]The experiment was conducted to study the regulatory effect of genistein on the reproductive performance of breeder hens during the late laying period,in an effort to lay the foundation for the development of feed additives for breeder hens.[Methods]18058-week-old Qiling breeder hens with similar body weight and physiological status were randomly divided into three groups:control group,low dose genistein(L-Gen)group and high dose genistein(H-Gen)group,with 5 replicate per group.The preparation period lasted for 2 weeks,while the formal experimental period lasted for 4 weeks.Hens in all groups were fed with the corn soybean meal basal diet for 2-week preparation period.During the formal experimental period,the control group was fed with the basal diet.The L-Gen and H-Gen groups were fed with 20 and 40 mg·kg^(-1) genistein,respectively.[Results]The results showed that adding 20 mg·kg^(-1) genistein in the diet significantly increased the average egg weight,eggshell thickness and GnRH mRNA expression in the hypothalamus(P<0.05);40 mg·kg^(-1) genistein treatment significantly increased the egg production rate and average egg weight in the 3rd and 4th weeks of the experiment,and the increased level of estrogen and progesterone in the serum(P<0.05).In addition,the egg yolk color grade and eggshell strength of L-Gen and H-Gen groups were higher than those of the control group(P<0.05).Furthermore,genistein treatment significantly increased the SOD activity and T-AOC content(P<0.05),and up-regulated the mRNA expression of ERαand FSHR in the yolk follicle(P<0.05).[Conclusions]In conclusion,genistein can activate the hypothalamus-pituitary-gonad axis,enhance the antioxidant capability and reproductive hormone secretion of the ovary,and improve the reproductive performance of breeder hens in the late laying period.
作者
魏全伟
吕增鹏
胡晨辉
戴宏建
李思梦
孙涛
石放雄
WEI Quanwei;Lü Zengpeng;HU Chenhui;DAI Hongjian;LI Simeng;SUN Tao;SHI Fangxiong(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Animal Husbandry and Veterinary Service Center of Lingcheng District of Dezhou City,Dezhou 253500,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2021年第5期936-942,共7页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(31972565)
中国高校博士后基金项目(2019M651867)
江苏省农业自主创新项目[CX(18)2002]。
关键词
种母鸡
染料木黄酮
繁殖性能
生殖激素
蛋品质
抗氧化
breeder hens
genistein
reproductive performance
hormones
egg quality
antioxidation
