摘要
建立普通产酮基古龙酸菌的高通量筛选方法,以实现快速检出生长快、高产2-酮基-L-古龙酸(2-KGA)的菌株。在液体和固体培养基中分别添加混合指示剂,根据颜色变化筛选产酸相对较高的菌株;采用离心管组培养技术进行大批量培养检测。实验结果表明:固体平板上单菌落周围培养基的变色情况、变色圈大小、液体培养基的变色程度与普通产酮基古龙酸菌代谢产酸度存在一定的对应关系。通过目测可直观检出生长快、产酸高的菌株,并由此筛选得到2-KGA的高产菌种K56-1。与对照菌种相比,K56-1发酵周期缩短约2.38 h,2-KGA生成速率提升7.23%。
A high-throughput screening method for common Ketogulonicigenium vulgare was established to achieve rapid detection strains with fast-growing and high-yield of 2-keto-L-gulonic acid(2-KGA).Mixed indicators were added to liquid and solid media respectively,and the strains with relatively high acid production were screened according to the color change;mass culture and detection were carried out by centrifugal tube group culture technology.The experimental results show that the discoloration condition,the size of the discoloration circle and the discoloration degree of the liquid medium around the single colony of the medium around a single colony on a solid plat were a certain corresponding relationship with the metabolic acidity of the common keto producing gulonic acid bacteria.The strains with fast growth and high acid production could be directly detected by visual inspection,and the 2-KGA high-yield strain K56-1 was screened.Compared with the control strain,the fermentation period of K56-1 was shortened by about 2.38 h,and the production rate of 2-KGA was increased by 7.23%.
作者
卢晓明
李晓东
Lu Xiaoming;Li Xiaodong(Shijiazhuang Weisheng Pharmaceutical(Shijiazhuang)Corporation Ltd.,Shijiazhuang 050035,China)
出处
《煤炭与化工》
CAS
2021年第8期127-131,共5页
Coal and Chemical Industry
