摘要
目的探讨下丘脑-垂体-肾上腺轴相关基因SKA2、AVPR1B、CRHR2基因位点的多态性与抑郁症患者自杀未遂行为的关联性及基因与环境因素的交互作用。方法选取自2017年3月至2018年8月因自杀就诊的抑郁症患者61例(病例组),同期选取与病例组年龄、性别、受教育程度匹配人员57例(对照组)。使用Snapshot分型技术对病例组和对照组进行基因型检验,采用Barratt冲动量表(Barratt impulsiveness scale,BIS-Ⅱ)、生活事件量表(life event scale,LES)分别评估个体的冲动特质及其过去1年的精神压力。使用SPSS 22.0软件,采用卡方检验、独立样本t检验进行组间比较;应用广义多因子降维法进行基因-环境交互作用分析。结果自杀未遂组BIS-Ⅱ总分和LES总分[分别为(65.05±11.14)分,(34.16±27.23)分]均高于对照组[分别为(53.30±9.07)分,(11.67±12.64)分],差异具有统计学意义(均P<0.05);两组SKA2和CRHR2基因的基因型与等位基因频率分布均差异无统计学意义(P>0.05);两组AVPR1B基因rs28373064的基因型和等位基因频率分布差异均具有统计学意义(χ2=5.763,4.279,均P<0.05),经Bonferroni校正后的分布差异无统计学意义(P>0.05)。GMDR中最优交互模型为AVPR1B基因rs28373064位点与冲动特质、生活事件构建的三阶模型,检验样本准确度最高0.789,模型差异具有统计学意义(P=0.001)。在多个遗传模式中,AVPR1B基因rs28373064位点与抑郁症患者自杀未遂行为存在关联[显性模式:A/G-G/G(OR=0.38,95%CI=0.16~0.88,P=0.021);超显性模式:A/G(OR=0.36,95%CI=0.15~0.87,P=0.019);累加模式:A/A、A/G及G/G(OR=0.44,95%CI=0.20~0.96,P=0.034)]。结论AVPR1B基因rs28373064多态性与抑郁症患者的自杀未遂行为相关,该位点AA基因型携带者在生活事件及冲动特质的影响下更容易发生自杀行为。
Objective To investigate the association between the polymorphisms of the hypothalamus pituitary adrenal axis related genes SKA2,AVPR1B,CRHR2 and suicide attempts in patients with depression,and the interaction between the genes and environmental factors.Methods From March 2017 to August 2018,sixty-one patients with depression who were hospitalized for suicide were selected(case group),and 57 subjects matched with the age,gender and education level of the case group(control group)were selected in the same period.Snapshot genotyping technique was used to test the genotypes of case group and control group.Barratt impulsivity scale(BIS-Ⅱ)and life event scale(LES)were used to assess the impulsive traits and mental stress of individuals in the past year.Chi-square test and independent sample t-test were used for inter group comparison by SPSS 22.0 software.Gene-environment interaction was analyzed by the generalized multi factor dimensionality reduction.Results The total scores of BIS-Ⅱand LES in case group(65.05±11.14,34.16±27.23)were higher than those in the control group(53.30±9.07,11.67±12.64),the differences were statistically significant(both P<0.05).There was no significant difference in gene frequency and allele frequency of SKA2 and CRHR2 between the two groups(P>0.05).The genotype frequency and allele frequency of rs28373064 of AVPR1B gene were significantly different between the two groups(χ2=5.763,4.279,both P<0.05),but there was no significant difference after Bonferroni correction(P>0.05).The best interaction model in GMDR was the third-order model constructed by rs28373064 of AVPR1B gene,impulsive traits and life events,with the highest accuracy of 0.789 for sample test(P=0.001).In multiple genetic models,rs28373064 of AVPR1B gene was associated with attempted suicide behavior in patients with depression(dominant model:A/G-G/G(OR=0.38,95%CI=0.16-0.88,P=0.021),overdominant model:A/G(OR=0.36,95%CI=0.15-0.87,P=0.019),log-additive model:A/A,A/G and G/G(OR=0.44,95%CI=0.20-0.96,P=0.034)).Conclusion r
作者
邱小英
瞿铭
张琳娜
褚成静
Qiu Xiaoying;Qu Ming;Zhang Linna;Chu Chengjing(Department of Psychology,School of Huamanities and Management(Research Center for Quality of Life and Applied Psychology,Key Laboratory for Quality of Life and Psychological Assessment and Intervention),Guangdong Medical University,Dongguan 523808,China;Department of Psychiatry,Kuitun Hospital of Ili Kazakh Autonomous Prefecture,Ili Kazak Autonomous Prefecture 833200,China;Brain Hospital Affiliated to Guangzhou Medical University,Guangzhou 510370,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2021年第8期694-700,共7页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目(81101021)。
