摘要
目的:探讨高迁移率族蛋白B1(HMGB1)基因对高糖诱导的血管内皮细胞损伤的影响及作用机制。方法:培养人血管内皮细胞,实验分为NG组(5.5 mmol/L葡萄糖处理)、HG组(33.3 mmol/L葡萄糖处理)、HG+si-NC组(转染si RNA阴性对照及33.3 mmol/L葡萄糖处理)和HG+si-HMGB1组(转染HMGB1 si RNA及33.3 mmol/L葡萄糖处理)。比较各组细胞HMGB1、p65和IκB-α蛋白表达,细胞活力、氧化应激及炎症反应水平差异。结果:与NG组比,HG组HMGB1 mRNA和蛋白表达升高,细胞活力降低,LDH和ROS升高,SOD降低,ICAM-1、MCP-1和IL-6水平升高,p65表达上调,IκB-α表达下调(P<0.05)。与HG+si-NC组比,HG+si-HMGB1组HMGB1 mRNA和蛋白的表达降低,细胞活力升高,LDH和ROS降低,SOD升高,ICAM-1、MCP-1和IL-6降低,p65表达下调,IκB-α表达上调(P<0.05)。结论:抑制HMGB1表达能提高血管内皮细胞的活力,减轻氧化损伤及炎症反应,其作用机制可能与抑制NF-κB信号通路的激活有关。
Objective:To investigate the effect of high mobility group box 1(HMGB1)gene on high glucose-induced vascular endothelial cell injury and its mechanism.Methods:Human vascular endothelial cells were cultured and divided into NG group(5.5 mmol/L glucose treatment),HG group(33.3 mmol/L glucose treatment),HG+si-NC group(transfected siRNA negative control and 33.3 mmol/L glucose treatment)and HG+si-HMGB1 group(transfected HMGB1 siRNA and 33.3 mmol/L glucose treatment).HMGB1,p65 and IκB-α protein expression,cell viability,oxidative stress and inflammatory response levels were compared among the groups.Results:Compared with those in the NG group,HMGB1 mRNA and protein expression were increased,cell viability was decreased,LDH and ROS were increased,SOD was decreased,ICAM-1,MCP-1 and IL-6 levels were increased,p65 expression was up-regulated,and IκB-α expression was down-regulated in the HG group(P<0.05).Compared with those in the HG+si-NC group,HMGB1 mRNA and protein expression were decreased,cell viability was increased,LDH and ROS were decreased,SOD was increased,ICAM-1,MCP-1 and IL-6 were decreased,p65 expression was down-regulated,and the IκB-α expression was up-regulated in the HG+si-HMGB1 group(P<0.05).Conclusion:Inhibition of HMGB1 expression can increase the viability of vascular endothelial cells,reduce oxidative damage and inflammatory response of vascular endothelial cells,and the mechanism may be associated with the inhibition of NF-κB signaling pathway activation.
作者
牛少辉
熊海燕
栗媛
Niu Shaohui;Xiong Haiyan;Li Yuan(Department of Cardiovascular Medicine,Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450000,China)
出处
《现代临床医学》
2021年第5期338-341,共4页
Journal of Modern Clinical Medicine
