摘要
目的探讨长链非编码RNA-癌基因SEI1-1(lnc-SERTAD1-1)对结直肠癌增殖、迁移及预后的影响。方法选取125例结直肠癌患者的标本,检测癌组织和癌旁正常组织中lncSERTAD1-1的表达水平,分析lnc-SERTAD1-1与临床病理特征的相关性,分析lnc-SERTAD1-1对结直肠癌预后的影响。检测正常人结肠组织细胞CCD-18Co与人结直肠癌细胞HCT15中lncSERTAD1-1的表达。慢病毒转染构建含有目的基因lnc-SERTAD1-1过表达的HCT15(HO)及含有空白载体质粒的HCT15(HOC),检测其lnc-SERTAD1-1以及SERTAD1蛋白的表达,并检测lnc-SERTAD1-1对结直肠癌细胞增殖和迁移能力的影响。结果与癌旁正常组织比较,lncSERTAD1-1在结直肠癌组织(0.002198±0.000499 vs.0.002998±0.000392,P<0.001)和癌细胞(0.000123±0.000010 vs.0.000182±0.000012,P=0.004)中呈低表达水平;其表达高低与结直肠癌患者的肿瘤部位、肿瘤大小及肿瘤的大体分型相关(P均<0.05)。在125例结直肠癌患者中,lnc-SERTAD1-1高表达(≥0.000970)是其术后总生存及无病生存的独立保护因素(总生存HR=0.228,95%CI:0.107~0.485,P<0.001;无病生存HR=0.228,95%CI:0.103~0.506,P<0.001)。体外实验显示lnc-SERTAD1-1表达上调能抑制结直肠癌细胞的增殖和迁移(P均<0.05)。结论lnc-SERTAD1-1通过抑制结直肠癌细胞的增殖和迁移发挥抑癌基因的作用,是结直肠癌重要的预后影响因素。
Objective To evaluate the effect of the long non-coding RNA(lncRNA)-SERTAD1-1(lnc-SERTAD1-1)on the proliferation,migration and prognosis of colorectal cancer(CRC).Methods A total of 125 samples were collected from CRC patients.The expression levels of lnc-SERTAD1-1 in the cancer tissues and adjacent normal tissues were measured.The correlation between lnc-SERTAD1-1 and clinicopathological characteristics was analyzed.The effect of lnc-SERTAD1-1 upon clinical prognosis of CRC patients was evaluated.The expression levels of lnc-SERTAD1-1 in normal human colon tissue cell CCD-18Co and human CRC cell line HCT15 were determined.HCT15 cells(HO)containing the overexpression of the target gene lnc-SERTAD1-1 and HCT15 cells(HOC)containing an empty vector plasmid were constructed by using lentiviral transfection.The expression levels of lnc-SERTAD1-1 and SERTAD1 protein were determined.The effect of lnc-SERTAD1-1 upon the proliferation and migration ability of CRC cells was assessed.Results Compared with adjacent normal tissues,lnc-SERTAD1-1 was lowly expressed in the CRC tissues and cancer cells(cancer tissues:0.002198±0.000499 vs.0.002998±0.000392,P<0.001;cancer cells:0.000123±0.000010 vs.0.000182±0.000012,P<0.004).The expression level of lnc-SERTAD1-1 was significantly correlated with the location,size and general classification of the tumor in CRC patients(all P<0.05).Among 125 CRC patients,high expression of lnc-SERTAD1-1(≥0.000970)was an independent protective factor of the overall survival(OS)and disease-free survival(DFS)of CRC patients(HR=0.228,95%CI:0.107-0.485,P<0.001 for OS;HR=0.228,95%CI:0.103-0.506,P<0.001 for DFS).In vitro experiment revealed that up-regulation of lnc-SERTAD1-1 expression significantly inhibited the proliferation and migration of CRC cells(both P<0.05).Conclusion lnc-SERTAD1-1 plays the role of tumor suppressor in CRC via suppressing cell proliferation and migration,which is a critical prognostic factor of CRC.
作者
陈志健
缪禄声
袁浩南
张旺发
孔连广
魏宜胜
Chen Zhijian;Miu Lusheng;Yuan Haonan;Zhang Wangfa;Kong Lianguang;Wei Yisheng(Department of Gastroenterology&Laboratory of Surgery,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China)
出处
《新医学》
CAS
2021年第9期651-658,共8页
Journal of New Medicine
基金
国家自然科学基金(81672436)
广州市科技创新委员会(201804010072)
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