摘要
以不同产地的12批地锦草样本为试验材料,采用80%(体积分数)甲醇溶液超声处理45 min,在检测波长254 nm、柱温30℃的条件下,采用超高效液相色谱-三重四极杆飞行时间质谱(UHPLC-Q-TOF-MS)技术,结合多重质量亏损(MMDF)和动态背景扣除(DBS),快速分析地锦草中化学成分。以鞣花酸峰作为参照峰,基于UHPLC技术与指纹图谱相似度软件建立不同产地的12批地锦草的UHPLC指纹图谱。结果表明,UHPLC-Q-TOF-MS快速鉴定出地锦草中37种化学成分;在建立的不同产地的12批地锦草UHPLC指纹图谱中,通过对照品比对指认了16个共有峰中的6个共有峰,分别为没食子酸(峰1)、鞣花酸(峰4)、槲皮素(峰13)、木犀草素(峰14)、芹菜素(峰15)、山奈酚(峰16),12批地锦草UHPLC指纹图谱相似度结果为0.841~0.985。
The samples of 12 batches of Euphorbia humifusae Willd.from different habitats as experimental materials,were treated with ultrasonic for 45 min in 80%(φ)methanol solution.Under the conditions of the detection wavelength of 254 nm and column temperature of 30℃,UHPLC-Q-TOF-MS combined with MMDF and DBS was used for rapidly analyzing the chemical constituents in Euphorbia humifusae Willd..Using ellagic acid peak as the reference peak,UHPLC fingerprints of 12 batches of Euphorbia humifusae Willd.from different habitats were established based on UHPLC and fingerprint similarity software.The results showed that 37 chemical constituents in Euphorbia humifusae Willd.were rapidly identified by UHPLC-Q-TOF-MS.In UHPLC fingerprints of 12 batches of Euphorbia humifusae Willd.from different habitats,6 peaks of the 16 common peaks were identified by comparison with reference substances,which were gallic acid(peak 1),ellagic acid(peak 4),quercetin(peak 13),luteolin(peak 14),apigenin(peak 15)and kaempferol(peak 16).The UHPLC fingerprint similarity of 12 batches of Euphorbia humifusae Willd.was in the range of 0.841-0.985.
作者
冯光维
黄春丽
许义红
龚元
魏学军
韩忠耀
FENG Guangwei;HUANG Chunli;XU Yihong;GONG Yuan;WEI Xuejun;HAN Zhongyao(Qiannan Medical College for Nationalities,Duyun 558000,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2021年第8期714-720,共7页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
黔南民族医学高等专科学校科研基金资助项目(qnyz202002)。
