摘要
【目的】评价6种抗氧化剂对单萜烯类化合物稳定性的影响及其在野外条件下单萜烯对松墨天牛Monochamus alternatus Hope成虫的诱杀效果。【方法】采用水浴锅加热加速氧化法,以气相色谱法测定含量,研究不同抗氧化剂对α-蒎烯、β-蒎烯和月桂烯稳定性的影响,并进行林间松墨天牛的诱捕试验。【结果】在供试的6种抗氧化剂中,TBHQ对单萜烯的保护效果最显著(P<0.05)。浓度对单萜烯的保护效果影响显著(F=6.631,P=0.007),其中浓度为0.03%的保护效果最显著(P<0.05)。抗氧化剂对α-蒎烯、β-蒎烯和月桂烯的保护效果存在显著差别(F=3.231,P=0.031),抗氧化剂对β-蒎烯的保护效果最显著(P<0.05)。野外诱捕实验表明添加抗氧化剂0.03%TBHQ的单萜烯(α-蒎烯∶β-蒎烯∶月桂烯=50∶40∶10)对松墨天牛野外诱捕持效期明显延长,持效期延长不少于30 d。【结论】添加抗氧化剂可保护单萜烯,维持单萜烯在野外条件下的稳定性,保持引诱剂成分的稳定性与持效性,延长单萜烯类昆虫引诱剂在野外诱捕害虫的持效期。
[Objectives]To investigate the effects of six antioxidants on the stability of monoterpene lures used to trap adult Monochamus alternatus Hope in the field.[Methods]The effects of different antioxidants on the stability ofα-pinene,β-pinene,and myrcene were determined by gas chromatography using the water bath heating and accelerated oxidation methods.A trapping assay of M.alternatus was carried out in forest.[Results]Of the 6 antioxidants tested,TBHQ had the most significant protective effect on monoterpenes(P<0.05).The concentration of TBHQ significantly affected monoterpene stability(F=6.631,P=0.007);the optimal concentration was 0.03%(P<0.05).There were significant differences in the stability of the three monoterpenesα-pinene,β-pinene,and myrcene even with the same antioxidant(F=3.231,P=0.031);α-pinene was the most stable(P<0.05).Field trapping results indicate that an olfactory lure comprised of 0.03%TBHQ and a ratio ofα-pinene:β-pinene:myrtene of 50∶40∶10 extended the effectiveness of lures in forest by at least 30 days.[Conclusion]Addition of antioxidant to protect monoterpenes significantly extends the life of monoterpene olfactory attractants used to trap M.alternatus in forest.
作者
吴华
戴建青
陈大嵩
黄鸿
WU Hua;DAI Jian-Qing;CHEN Da-Song;HUANG Hong(Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangdong Engineering Research Center for Mineral Oil Pesticides,Institute of Zoology,Guangdong Academy of Sciences,Guangzhou 510260,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2021年第4期894-899,共6页
Chinese Journal of Applied Entomology
基金
广东省科学院科技发展专项(2018GDASCX-0107,2019GDASYL-0302007)。
