摘要
目的检测食管鳞癌(ESCC)患者癌组织中微小RNA-206(miR-206)、蛋白酪氨酸磷酸酶1B(PTP1B)表达情况,并探讨二者与临床病理特征及预后的关系。方法选取2013年6月至2016年2月本院收治的80例ESCC患者癌组织为ESCC组,另选取距癌组织边缘>5 cm的对应癌旁组织标本为对照组。采用实时荧光定量PCR测定miR-206、PTP1B mRNA表达水平;采用免疫组织化学法测定PTP1B蛋白表达情况;分析miR-206、PTP1B蛋白表达与临床病理特征及ESCC患者预后的关系;COX法分析影响ESCC患者预后的因素。结果与对照组比较,ESCC组癌组织中miR-206水平显著降低(P<0.05),PTP1B mRNA水平、PTP1B蛋白阳性表达率显著升高(P<0.05)。ESCC患者癌组织miR-206、PTP1B mRNA表达呈负相关(P<0.05)。miR-206、PTP1B蛋白表达与ESCC患者TNM分期、组织分化程度、淋巴结转移有关(P<0.05),与年龄、肿瘤大小、肿瘤位置、浸润程度无关(P>0.05)。miR-206低表达患者5年生存率12.20%(5/41)显著低于miR-206高表达患者77.14%(27/35)(P<0.05),PTP1B蛋白阳性患者5年生存率29.31%(17/58)显著低于PTP1B蛋白阴性患者83.33%(15/18)(P<0.05)。TNM分期为Ⅲ+Ⅳ期、低分化程度、淋巴结发生转移、miR-206低表达、PTP1B蛋白阳性均是影响ESCC患者预后的危险因素(P<0.05)。结论miR-206低表达、PTP1B蛋白阳性表达可能与ESCC发生发展有关,且可一定程度评估患者预后,对指导临床治疗方面有一定参考意义。
Objective To detect the expressions of microRNA-206(miR-206)and protein tyrosine phosphatase 1B(PTP1B)in esophageal squamous cell carcinoma(ESCC)tissues,and to explore their relationships with clinicopathological features and the prognosis.Methods A total of 80 patients with ESCC from June,2013 to February,2016 in our hospital were selected as the ESCC group,and corresponding paracancerous tissue samples which had a>5 cm distance from the edge of cancer tissue were selected as the control group.The mRNA expression levels of miR-206 and PTP1B were detected by real-time fluorescence quantitative PCR and the expression of PTP1B protein was detected by immunohistochemistry.The relationships between the expressions of miR-206 and PTP1B protein and clinicopathological features and the prognosis of ESCC patients were analyzed.COX method was applied to analyze the prognostic factors of ESCC patients.Results Compared with those in the control group,the level of miR-206 in the ESCC group was significantly lower(P<0.05),while the level of PTP1B mRNA and positive expression rate of PTP1B protein were significantly higher(P<0.05).The expressions of miR-206 and PTP1B mRNA were negatively correlated in ESCC(P<0.05).The expressions of miR-206 and PTP1B was correlated with TNM stage,histological differentiation and lymph node metastasis(P<0.05),but not with age,tumor size,tumor location and invasion(P>0.05).The 5-year survival rate of patients with low expression of miR-206 was 12.20%(5/41),which was significantly lower than 77.14%(27/35)of patients with high expression of miR-206(P<0.05).The 5-year survival rate of patients with positive PTP1B protein was 29.31%(17/58),which was significantly lower than 83.33%(15/18)of patients with negative PTP1B protein(P<0.05).TNM stageⅢ+Ⅳ,low differentiation degree,lymph node metastasis,low expression of miR-206 and positive PTP1B protein were all risk factors for the prognosis of ESCC patients(P<0.05).Conclusion Low expression of miR-206 and positive expression of PTP1B protein may be r
作者
张军
杨谦
马玉泉
谭雪敏
ZHANG Jun;YANG Qian;MA Yuquan;TAN Xuemin(Department of Thoracic Surgery,Handan Central Hospital,Handan 056000,China)
出处
《标记免疫分析与临床》
CAS
2021年第8期1338-1343,共6页
Labeled Immunoassays and Clinical Medicine
基金
河北省医学科学研究课题计划项目(编号:20191825)。
