摘要
目的探讨外周血微小核糖核酸-199a(miRNA-199a)与特发性肺动脉高压(IPAH)预后的相关性。方法选取2015年11月—2017年11月河南省人民医院收治的IPAH患者42例(IPAH组),另选取同期性别、年龄匹配的健康人40例(对照组)作为研究对象,采用逆转录聚合酶链反应(RT-PCR)检测两组外周血miRNA-199a表达水平;采用Pearson相关分析法对IPAH组miRNA-199a与心导管检查指标(肺动脉平均压、肺血管阻力和肺血管阻力指数)进行相关性分析;应用ROC曲线评价血浆miRNA-199a表达水平对IPAH患者预后的预测价值。结果IPAH组外周血miRNA-199a水平显著低于对照组(P<0.05),差异有统计学意义;Pearson相关分析结果显示miRNA-199a表达水平与肺动脉平均压、肺血管阻力和肺血管阻力指数均呈显著负相关(r=-0.509,P<0.05;r=-0.637,P<0.05;r=-0.591,P<0.05);ROC曲线分析结果显示,外周血miRNA-199a预测IPAH患者预后不良的最佳截断值为1.36,预测的AUC为0.834(95%CI 0.734~0.908),与AUC=0.5比较,差异有统计学意义(Z=6.430,P<0.001)。结论IPAH患者外周血miRNA-199a表达水平明显下调,其对IPAH患者预后具有较好的预测价值。
Objective To explore the correlation of serum microRNA-199a(miRNA-199a)with prognosis of idiopathic pulmonary arterial hypertension(IPAH).Methods 42 patients with IPAH(IPAH group)admitted to our hospital from November 2015 to November 2017 and contemporary 40 healthy people(control group)with matched gender and age were enrolled as research objects.The expression level of miRNA-199a in peripheral blood was detected by RT-PCR.Pearson correlation analysis was used to analyze the relationship between miRNA-199a and cardiac catheter examination indexes[mean pulmonary artery pressure(mPAP),pulmonary vascular resistance(PVR),pulmonary vascular resistance index(PVRi)]in IPAH group.The predictive value of plasma miRNA-199a in prognosis of IPAH patients was evaluated by ROC curves.Results miRNA-199a level in peripheral blood was obviously lower in IPAH group than control(P<0.05).Results of Pearson correlation analysis showed miRNA-199a level was negatively correlated with mPAP,PVR and PVRi(r=-0.509,P<0.05;r=-0.637,P<0.05;r=-0.591,P<0.05).ROC curve analysis showed the best cut-off value and AUC of miRNA-199a in peripheral blood for predicting poor prognosis of IPAH patients were 1.36 and 0.834(95%CI 0.734~0.908)respectively.Differences were statistically significant compared with AUC=0.5(Z=6.430,P<0.001).Conclusion The expression level of miRNA-199a in peripheral blood is significantly down-regulated in IPAH patients,which could be of good predictive value in prognosis of IPAH.
作者
宋玮
齐咏
陈卓昌
SONG Wei;QI Yong;CHEN Zhuochang(Department of Respiratory and Critical Diseases Medicine,Henan People's Hospital,Zhengzhou 450000,Henan,China)
出处
《心血管病学进展》
CAS
2021年第8期763-766,共4页
Advances in Cardiovascular Diseases
基金
2019年河南省医学科技攻关计划省部共建项目(YXKC2020005)。
