摘要
提出三维荧光二阶校正法,用于环境水样中氟喹诺酮类抗生素培氟沙星和氧氟沙星的同时定量分析。即在激发波长为230~400 nm,发射波长为360~580 nm范围内,测定样品(包含校正样、验证样、测试样)的三维荧光光谱,构建三维荧光响应数据阵;经数据处理后,采用交替三线性分解算法进行分解,得到与物质相关的相对激发、相对发射和相对浓度谱信息;通过对浓度信息进行单变量校正,获得校正曲线,进一步预测真实浓度。结果表明,培氟沙星和氧氟沙星的平均回收率分别为(101.1±5.3)%,(99.7±4.7)%,检测限分别为2.14,4.34 ng/mL,定量限分别为6.49,13.16 ng/mL。三维荧光二阶校正法可以在未知干扰物共存下,同时定量分析环境水样中培氟沙星和氧氟沙星,实现二阶优势。
A novel method was proposed for the simultaneous determination of pefloxacin and ofloxacin in the environmental water using three-dimension fluorescence spectra coupled with second-order calibration method.All the samples were measured at excitation wavelengths from 230 nm to 400 nm and the emission wavelengths from 360 nm to 580 nm.A three-way data array was generated and then subjected to dealing with the alternating trilinear decomposition algorithm,thus resulting in relative excitation,emission and concentration profiles related to target components and unknown interference(s).These results suggested that a univariate calibration curve associated with component of interest was obtained by the relative concentrations vs the actual concentrations in calibration samples,and further used to predict content in test samples.The average recoveries of pefloxacin and ofloxacin were(101.1±5.3)%and(99.7±4.7)%,with limit of detection being 2.14,4.34 ng/mL,and limit of quantitation 6.49,13.16 ng/mL,respectively.The proposed method could be applied to simultaneous determination of pefloxacin and ofloxacin in the environmental water even in the presence of unknown interference(s),and realize the second-order advantage.
作者
谢丽霞
李志能
汤建新
谭益民
郑思乡
廖晓珊
XIE Lixia;LI Zhineng;TANG Jianxin;TAN Yimin;ZHENG Sixiang;LIAO Xiaoshan(College of Life Sciences and Chemistry,Hunan University of Technology,Zhuzhou Hunan 412007,China;Hunan Provincial Engineering Research Center of Lily Germplasm Resource Innovation and Deep Processing,Zhuzhou Hunan 412007,China;Agricultural Science Research Institute of Zhuzhou,Zhuzhou Hunan 412007,China)
出处
《包装学报》
2021年第4期24-31,共8页
Packaging Journal
基金
国家自然科学基金资助项目(21806034)
湖南省教育厅科学研究基金资助项目(19C0603)
株洲市科技人才托举工程基金资助项目(2019TJ-06)。
关键词
三维荧光
二阶校正
培氟沙星
氧氟沙星
未知干扰
three-dimension fluorescence
second-order calibration
pefloxacin
ofloxacin
unknown interference
