摘要
目的探讨利拉鲁肽减轻糖尿病大鼠脑缺血损伤的作用及机制。方法实验动物为SPF级SD雄性大鼠54只,分为假手术组(S组)、糖尿病合并脑缺血组(Di组)、利拉鲁肽干预糖尿病合并脑缺血组(Li组),每组18只。采用神经功能缺损评分对各组大鼠的神经功能进行评估;采用2,3,5-氯化三苯基四氮唑(TTC)染色法检测脑梗死面积;采用过氧化氢还原法检测脑组织中的髓过氧化物酶(MPO)活性;采用酶联免疫吸附试验(ELISA)检测脑组织中的IL-1β水平;采用Western blot检测脑组织核转录因子-κB(NF-κB)和肿瘤坏死因子-α(TNF-α)的蛋白表达水平。结果神经功能缺损评分显示:S组、Li组和Di组分别为0、(2.57±0.64)、(3.50±0.55)分,Di组和Li组的评分均高于S组(P<0.05),Li组的评分低于Di组(P<0.05)。TTC染色显示:S组、Li组和Di组的梗死面积百分比分别为0、(29.44±2.52)%、(36.58±4.85)%,Di组和Li组的梗死面积百分比均高于S组(P<0.05),Di组高于Li组(P<0.05);MPO活性的测定:S组、Di组和Li组分别为(0.067±0.003)、(0.973±0.049)、(0.549±0.420)U/mL,Di组和Li组均高于S组(P<0.05),Li组比Di组显著降低(P<0.05)。IL-1β水平的测定:S组、Di组和Li组分别为(35.43±2.35)、(72.42±6.13)、(56.94±2.15)pg/mL,Di组和Li组均高于S组(P<0.05),Li组比Di组显著降低(P<0.05)。NF-κB、TNF-α蛋白表达的相对水平:Di组和Li组均高于S组(P<0.05),Li组比Di组显著降低(P<0.05)。结论利拉鲁肽具有减轻糖尿病大鼠局灶性脑缺血损伤的作用,其机制与降低MPO活性、降低NF-κB及相关炎性因子TNF-α、IL-1β的表达有关。
Objective To investigate the effect and mechanism of liraglutide in reducing cerebral ischemic damage in diabetic rats.Methods The experimental animals were 54 SPF male SD rats,which were divided into sham operation group(S group),diabetes combined with cerebral ischemia group(Di group),and liraglutide intervention diabetes combined with cerebral ischemia group(Li group),n=18 in each group.The neurological deficit score was used to evaluate the neurological function of each group.The 2,3,5-triphenyltetrazolium chloride(TTC)staining method was used to detect the area of cerebral infarction;the hydrogen peroxide reduction method was used to detect the myeloperoxidase(MPO)activity in brain tissue;enzyme-linked immunosorbent assay(ELISA)was used to detect the level of interleukin-1β(IL-1β)in brain tissue;Western blot was used to detect nuclear transcription factor-κB(NF-κB)in brain tissue and tumor necrosis factor-α(TNF-α)protein expression level.Results The neurological deficit scores showed that the scores of S group,Li group and Di group were 0,(2.57±0.64),(3.50±0.55),respectively.The scores of Di group and Li group were higher than those of S group(P<0.05),The score of Li group was lower than that of Di group(P<0.05).TTC staining showed that the infarct area percentages of S group,Li group and Di group were 0,(29.44±2.52)%,(36.58±4.85)%,respectively,and the percentage of infarct areas of Di group and Li group were higher than that of S group(P<0.05),the Li group is higher than that of Di group(P<0.05).Measurement of MPO activity:the MPO activity of S group,Di group and Li group were(0.067±0.003),(0.973±0.049),(0.549±0.420)U/mL,respectively;the Di and Li group were higher than S group(P<0.05),the Li group was significantly lower than the Di group(P<0.05).Determination of IL-1βlevels:the levels of the S group,Di group and Li group were(35.43±2.35),(72.42±6.13),(56.94±2.15)pg/mL,respectively;the Di group and Li group were higher than S group(P<0.05),the Li group was significantly lower than the Di g
作者
勿坡巫且
邓才洪
李伍基
杨林
沈树安
白子格
WUPO Wuqie;DENG Caihong;LI Wuji;YANG Lin;SHEN Shu′an;BAI Zige(Department of Neurology,the Second People′s Hospital of Liangshan,Xichang,Sichuan 615000,China;Department of Neurology,the Affiliated Hospital&Clinical Medical College of Chengdu University,Chengdu,Sichuan 610000,China)
出处
《国际检验医学杂志》
CAS
2021年第17期2105-2108,2112,共5页
International Journal of Laboratory Medicine
基金
成都大学自然科学重点项目(2020YZ203)。
