摘要
【背景】自2010年以来,我国鸡群中滑液囊支原体(Mycoplasma synoviae,MS)的感染率不断增高,目前MS已广泛存在于我国不同的鸡群中,包括蛋鸡、种鸡、白羽肉鸡和地方品种鸡等,其血清阳性率已超过40%,严重危害我国养鸡业,并造成了严重的经济损失。【目的】系统比较MS以不同感染途径对56日龄无特定病原体(Specific Pathogen Free,SPF)鸡的致病力。【方法】采用MS强毒FZ株以点眼、爪垫注射、胸部皮下注射、单次气管注射、连续3次气管注射等不同途径感染56日龄SPF鸡,观察感染后临床症状和解剖病理变化,检测感染后血清中的MS抗体,并且对气管组织进行病原再分离和组织病理学观察。【结果】MS的FZ株以不同途径感染SPF鸡后临床表现和发病率差异较大,爪垫注射和胸部皮下注射可导致100%的鸡发生爪垫肿大或胸部囊肿,而单次或连续3次气管注射可引起33%-50%的鸡发生严重气囊炎,点眼感染途径基本不能引起临床病理变化;爪垫肿大主要为肉芽组织增生和出现大量的黄色干酪样块状物质,胸部囊肿在囊肿部位有大量的血红色样液体和黄色干酪样块状物质;组织病理学结果显示连续3次气管注射方式感染更易对气管造成损伤,表现为气管黏膜固有层/黏膜下层发生轻微至轻度灶性炎细胞浸润,而爪垫肿大和胸部囊肿组织有大量的纤维组织和血管增生,同时伴有大量炎性细胞浸润;点眼和气管注射途径的气管MS病原再分离率可达100%,而胸部皮下注射或脚垫注射也可从气管中分离到MS病原;爪垫注射途径更易引起MS抗体转阳。【结论】系统比较了MS以不同感染途径对56日龄SPF鸡的致病性,并筛选出了相应的评价指征,成功建立了MS人工感染8周龄SPF鸡的发病模型,其中点眼感染途径和气管注射途径以气管病原再分离作为主要指征,辅以气囊炎进行评价,而胸部皮下注射和爪垫注射途径分别以�
[Background]The infection rate of Mycoplasma synoviae(MS)in chicken flocks has been increasing in China since 2010,and MS has been widely present in different flocks,including laying hens and/or breeders,white feather broilers and local breeds.The positive rate of MS antibody has exceeded 40%,which has seriously harmed Chinese chicken industry and caused serious economic losses.[Objective]The pathogenicity of MS to 56-day-old specific pathogen free(SPF)chickens with different infection routes was simultaneously compared.[Methods]The MS virulent FZ strain was used to infect 56-day-old SPF chickens by eye dropping,footpad injection,chest subcutaneous injection,single tracheal injection,and three consecutive tracheal injections.The clinical symptoms and anatomical pathological changes were observed from infection chickens,and MS antibody,re-isolation of pathogens from the trachea,and histopathological was determined at post-infection.[Results]The chicken infected MS FZ strain with different routes had different clinical symptoms and different morbidity.Footpad injection and chest subcutaneous injection could cause 100%of chickens exhibiting footpad swelling or chest cyst,while a single or three consecutive tracheal injection could cause 33%-50%of chickens exhibiting severe air sacculitis,the eye dropping infection had hardly caused clinical pathological changes;The footpad swelling showed proliferation of granulation tissue and a large amount of yellow cheese-like mass,and some blood-red liquid and yellow cheese-like mass were found in the chest cyst;Histopathological results showed that three consecutive tracheal injection were more likely to cause damage to the trachea,manifested mild inflammatory cell infiltration in the lamina propria/submucosa of the trachea,footpad swelling and chest cyst tissue had a lot of fibers tissue and blood vessel hyperplasia,accompanied by a large number of inflammatory cell infiltration.It was found that MS re-isolation from tracheal of birds challenged by eye dropping and tracheal i
作者
侯博
王晨燕
邵国青
HOU Bo;WANG Chenyan;SHAO Guoqing(Fujian Animal Disease Control Technology Development Center,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2021年第8期2704-2713,共10页
Microbiology China
关键词
滑液囊支原体
致病力
感染模型
不同感染途径
Mycoplasma synoviae
pathogenicity
infection model
different infection routs
