摘要
目的:通过药效学指标检测,评价红花在活血化瘀方面的作用。方法:42只大鼠分为空白组、模型组、阿司匹林治疗组(15 mg·kg^(-1))、红花低、中、高剂量组(1000、2000、4000 mg·kg^(-1)),采用皮下注射肾上腺素结合冰水游泳复制大鼠急性血瘀模型,测定各组大鼠的血液流变学指标、凝血参数、血小板聚集率、血浆6-keto-PGF1α、血栓素B2(TXB2)和内皮型一氧化氮合酶(eNOS)含量。结果:红花高中低剂量组能不同程度降低血小板聚集率,延长凝血酶原时间(PT)和活化部分凝血酶原时间(APTT),降低全血粘度(WBV)、血浆粘度(PV),升高血清eNOS、6-keto-PGF1α,降低TXB2含量等。结论:红花通过恢复血小板聚集活性、改善凝血功能、血液流变性、血管内皮功能,抑制血栓形成等途径,对大鼠急性血瘀有较好的保护和治疗作用.
Objective:To evaluate the effect of safflower on promoting blood circulation and removing blood stasis by pharmacodynamic index test.Method:42 rats were divided into control group,model group,aspirin treatment group(15 mg·kg^(-1)),safflower low,medium and high dose groups(1000 mg·kg^(-1),2000 mg·kg^(-1),4000 mg·kg^(-1)).The acute blood stasis model was established by subcutaneous injection of adrenaline and swimming in ice water.The hemorheological indexes,coagulation parameters,platelet aggregation rate,the levels of plasma 6-Keto-PGF1α,TXB2 and eNOS were measured.Result:Safflower low,medium and high dose group can reduce platelet aggregation rate,prolong prothrombin time(PT)and activated partial prothrombin time(APTT),reduce whole blood viscosity(WBV)and plasma viscosity(PV),increase serum eNOS and 6-keto-pgfla,and reduce TXB2.Conclusion:Safflower has good protective and therapeutic effects on acute blood stasis in rats by restoring platelet aggregation activity,improving coagulation function,hemorheology,vascular endothelial function and inhibiting thrombosis.
作者
刘倩倩
王越
周婧
朱新龙
陈超
王升菊
裴瑾
LIU Qian-qian;WANG Yue;ZHOU Jing;ZHU Xin-long;CHEN Chao;WANG Sheng-ju;PEI Jin(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan)
出处
《中药与临床》
2021年第3期62-64,94,共4页
Pharmacy and Clinics of Chinese Materia Medica
基金
国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药人函[2019]41号)
四川省中医药管理局中医药重点学科建设项目(川中医药函[2020]84号)。
关键词
红花
急性血瘀
大鼠
凝血
活血化瘀
Safflower
acute blood stasis
rat
coagulation
blood circulation and stasis reduction
