摘要
目的:探究低强度聚焦超声刺激(LIFUS)对血管性痴呆(VD)大鼠工作记忆能力的影响,并从内侧前额叶皮层(mPFC)神经细胞存活和突触相关蛋白,如:N-甲基-D-天冬氨酸受体2B(NR2B)、突触素(SYP)重组蛋白、突触后密度蛋白-95(PSD-95)表达方面探讨影响机制。方法:随机将25只成年雄性Wistar大鼠,分为假手术组(sham)和模型组(model)(n=17)。通过双侧颈总动脉结扎手术(2-VO)建VD模型,术后14 d,将手术组存活的12只大鼠随机分为2-VO模型组(2-VO,n=6)和超声治疗组(2-VO+LIFUS,n=6)。2-VO+LIFUS组进行为期2周的超声刺激干预,刺激靶区为双侧mPFC,每天各刺激10 min。刺激参数为:中心频率=0.5 MHz;脉冲重复频率=2.0 kHz;周期数=150。同时sham组和2-VO组施加伪刺激。14 d后,行Y迷宫自发交替任务、延迟-非匹配样本任务,并通过Nissl染色和Western Blot检测mPFC神经细胞的存活及蛋白表达情况。结果:与sham组比较,2-VO组大鼠正确率明显降低,反应时间加长,尼氏体数量和NR2B蛋白的表达均显著下降。与2-VO组比较,2-VO+LIFUS组大鼠的自发交替率、正确率显著升高,反应时间缩短,尼氏体阳性表达数量明显增多,NR2B蛋白的表达升高。结论:LIFUS可明显改善2-VO大鼠的工作记忆功能,可能是与调控大鼠缺血后mPFC细胞存活、突触相关蛋白表达能力有关。
Objective: To examine the effects of low-intensity focused ultrasound stimulation (LIFUS) on working memory and medial prefrontal cortex (mPFC) neuronal cell survival and expression of synaptic related protein in vascular dementia (VD) rats. Methods: Adult male Wistar rats (n = 25) were randomly divided into the sham operation group (sham),and model group (model). The operation group underwent bilateral common carotid artery occlusion.Fourteen days after operation,the surviving rats were divided into 2-VO group (model) and 2-VO + LIFUS group (2-VO + LIFUS). For 2-VO + LIFUS group,LIFUS treatment was performed daily for 10 min on each side of mPFC. We used the following parameters: center frequency = 0. 5 MHz;pulse repetition frequency = 2. 0 kHz;the number of cycles= 150. The animals in both sham and 2-VO groups underwent the same procedures including anesthesia but without LIFUS. They were tested with Y maze spontaneous alternation task and delay-mismatched sample task 14 days after intervention. And the cell viability and expression of protein in mPFC were detected by Nissl’s staining and Western Blot.Results: Compared with sham group,the correct rate in 2-VO group was decreased;reaction time increased and the amount of Nissl bodies and the expression of NR2B decreased significantly in mPFC. Compared with 2-VO group,the spontaneous alternation rate and the correct rate of 2-VO + LIFUS group increased;reaction time decreased;the amount of Nissl bodies and the level of NR2B increased. Conclusion: LIFUS could significantly improve the working memory function of rats in VD,which was related to the regulation of mPFC cell survival and protein expression after ischemia.
作者
杨佳佳
王倩
王发颀
明东
Yang Jiajia;Wang Qian;Wang Faqi;Ming Dong(Academy of Medical Engineering and Translational Medicine,Tianjin University,Tianjin 300072,China;The College of Precision Instruments&Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)
出处
《神经解剖学杂志》
CSCD
2021年第4期392-398,共7页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(81871517)
国家重点研发项目(2017YFB1300302)。
关键词
血管性痴呆
低强度聚焦超声
工作记忆
大鼠
vascular dementia
low-intensity focused ultrasound
working memory
rat
