摘要
目的了解我院2017~2019年侵袭性真菌感染的病原谱及药敏情况,为临床真菌感染提供病原学诊断和合理应用抗真菌药物提供参考。方法对门诊及住院患者送检标本进行真菌培养、分离及鉴定,采用微量稀释法(ATB Fungus 3)进行药敏试验,检测假丝酵母菌属对抗真菌药物的敏感性。结果共分离出2862株真菌,包括假丝酵母菌属2440株(85.3%),曲霉菌属346株(12.1%),酵母属40株(1.4%),隐球菌属19株(0.7%)和毛霉菌科4株(0.1%)。侵袭性真菌感染以假丝酵母菌属为主,其中白色假丝酵母菌比例最高,占比为56.6%(1619/2862)。送检标本最多为痰液,其他依次为尿液、引流液、粪便、分泌物、胆汁和全血。真菌感染科室分布以肝胆外科、呼吸内科、感染科和重症医学科为主,占47.3%。真菌感染男性和女性比较差异无统计学意义(P>0.05)。随着年龄的增长,真菌感染的概率逐渐增加。药敏结果显示,假丝酵母菌属对抗真菌药物敏感性均较高,对两性霉素B最为敏感,敏感率为100%,对伊曲康唑的敏感性最低,平均敏感率为77.9%。结论近3年来我院真菌感染主要为假丝酵母菌属,以白色假丝酵母菌为主;最多的感染部位为呼吸系统;年龄越大,真菌感染的概率越大。假丝酵母菌属对抗真菌药物敏感性均较高,对两性霉素B最为敏感。
Objective To explore the pathogen spectrum and susceptibility of invasive fungal infection in our hospital from 2017 to 2019,so as to provide the basis for clinical fungal infection diagnosis and rational application of antifungal drugs.Methods The samples collected from outpatient and inpatients in our hospital were used for fungal culture,isolation and identification.The antimicrobial susceptibility of Candida was tested by using microdilution method(ATB Fungus 3).Results A total of 2862 strains of fungi were isolated,including 2440 strains of Candida(85.3%),346 strains of Aspergillus(12.1%),40 strains of yeast(1.4%),19 strains of Cryptococcus(0.7%)and 4 strains of Mucor(0.1%).The most invasive fungal infection was Candida,especially Candida albicans(56.6%,1619/2862).The most common specimen was sputum,followed by urine,drainage,feces,secretions,bile and blood.Fungal infection departments were mainly distributed in hepatobiliary surgery,respiratory medicine,infection department and central ICU,accounting for 47.3%of the total.There was no significant difference in fungal infection between men and women(P>0.05).With the increase of age,the probability of fungal infection increased gradually.Antimicrobial susceptibility testing showed that Candida had high susceptibility to antifungal drugs,all the fungal strains(100%)were susceptible to amphotericin B,but 77.9%susceptible to itraconazole(the lowest).Conclusion In the past 3 years,the main pathogenic fungi of invasive fungal infection in our hospital were Candida,of which Candida albicans was the prominent fungal pathogen,and the most infected site is respiratory system.The older the age,the higher the risk of fungal infection.Candida is highly susceptibility to antifungal drugs and amphotericin B is the most active antifungal agent in vitro.
作者
李芳
王静
李雯
袁晶
刘哲
张祎
曾晓艳
LI Fang;WANG Jing;LI Wen;YUAN Jing;LIU Zhe;ZHANG Yi;ZENG Xiaoyan(Department of Clinical Laboratory,the First Affiliated Hospital,Xi′an Jiaotong University,Xi′an 710061,China)
出处
《西北药学杂志》
2021年第4期674-677,共4页
Northwest Pharmaceutical Journal
基金
国家自然科学基金项目(编号:81201326)
陕西省重点研发计划项目(编号:2021SF-019)。
关键词
侵袭性真菌
病原谱
药敏试验
invasive fungal infection
pathogenic spectrum
drug susceptibility test
