摘要
基于淫羊藿成分多样,质控单一的问题,建立13个化学成分的一测多评的评价方法,进一步初探不同批次淫羊藿生品以及酒浸品的成分差异以及酒浸对成分的影响,为提升淫羊藿质量评控提供参考。采用超高效液相色谱构建不同批次淫羊藿的指纹图谱来评价其批次间的一致性,通过含量测定结果和热图分析淫羊藿在酒浸过程中的黄酮类成分的变化并初步探讨其变化的原因;然后选择《中国药典》质控成分淫羊藿苷为内参物,考察各化学成分在不同条件下的相对校正因子的稳定性,得到待测成分的相对校正因子,比较一测多评法(QAMS法)和外标法测定结果的差异,验证一测多评法的准确性。结果表明,不同批次淫羊藿药材均符合《中国药典》检测规定,同一产地的淫羊藿药材的指纹图谱的相似度较高,进一步分析发现,异戊烯基黄酮类成分可有效区分淫羊藿生品和酒浸品,可作为潜在的质量识别标志物,同时初步探讨了淫羊藿在酒浸过程中的糖苷水解过程;研究建立的测定淫羊藿中13种化学成分含量的一测多评法含量测定的准确性较高,耐用性较好,且在不同实验条件下重现性良好,2种分析方法所得结果无显著差异,对更加全面、快速、合理地评控淫羊藿的质量具有参考价值。
The quality control of Epimedii Folium, composed of diverse constituents, is single at present. In view of this, an eva-luation method of 13 chemical constituents based on quantitative analysis of multi-components by single marker(QAMS) was established to further explore the composition differences of raw products and alcohol extracts in different batches and the influence of alcohol extraction on the composition, so as to provide a reference for improving the quality evaluation and control of Epimedii Folium. The fingerprints of different batches of Epimedii Folium were constructed by ultra-high performance liquid chromatography(UPLC) to evaluate the inter-batch consistency. The changes of the flavonoids in Epimedii Folium during alcohol extraction were analyzed based on determined levels and heat map, and the reasons for the changes were preliminarily discussed. With icariin, the quality control component recorded in the Chinese Pharmacopoeia, as the internal reference, the stability of the relative correction factors of chemical components under different conditions was investigated to obtain the relative correction factors. Then the determination results of QAMS and the external standard method were compared to verify the accuracy of QAMS. The results revealed that all batches of Epimedii Folium met the requirements specified in the Chinese Pharmacopoeia, and the fingerprints of Epimedii Folium from the same place of origin exhibited a high similarity. Raw products and alcohol extracts of Epimedii Folium could be clearly distinguished by prenylated flavonoids, which are potential biomarkers for quality control. Additionally, the glycoside hydrolysis in the alcohol extraction was preliminarily explored. The QAMS method has good accuracy, durability, and repeatability in determining 13 chemical components in Epimedii Folium under different experimental conditions. No significant difference in the results obtained by the two methods was observed. This study can provide a reference for comprehensive, rapid and re
作者
张龙
李珊珊
白雪
李会芳
肖小河
赵旭
牛明
ZHANG Long;LI Shan-shan;BAI Xue;LI Hui-fang;XIAO Xiao-he;ZHAO Xu;NIU Ming(Shanxi University of Chinese Medicine,Jinzhong 030619,China;the Fifth Medical Center,General Hospital of the People's Liberation Army,Beijing 100039,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第11期2843-2851,共9页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707002)。
关键词
淫羊藿
一测多评
化学成分
质量评价
Epimedii Folium
quantitative analysis of multi-components by single marker(QAMS)
chemical composition
quality evaluation
