摘要
通过对大蒜(Allium sativum L.)不同发育时期鳞芽的徒手解剖和石蜡切片显微观察,鳞芽生长发育主要分为3个时期,即鳞芽分化前期、鳞芽分化期、鳞芽膨大期。鳞芽分化前期是叶原基的形成时期,叶原基具有典型的原套—原体结构。鳞芽分化期是小鳞芽的形成时期,小鳞芽形成位置与分生细胞团所在位置有关,已经形成的鳞芽在进行物质积累的同时其内部生长点还在继续分化。在鳞芽膨大期,分生组织细胞核中染色体变化活跃,分生细胞团内部有淀粉粒的积累,木质部具有发达的导管。对鳞芽分化期不同部位的切片进行PAS染色,发现细胞分裂旺盛的生长锥糖颗粒含量丰富,细胞质浓,染色较深,糖颗粒物质在分生细胞及其周围细胞之间存在着浓度梯度。
In the present study,the process of garlic(Allium sativum)clove development was observed and analyzed through freehand anatomy and paraffin embedded.The morphological observation showed that there were three stages,including the pre-differentiation stage,differentiationstage and expansion stage for clove development before mature.At the pre-differentiation stage,the leaf primordia with typical tunica-corpus structure was formed.At the differentiation stage,small clove formed.The location of small clove formation was related to the location of meristematic cell mass.The internal growth points of the formed scale clove continued to differentiate with the accumulation of clove material.At the expansion stage,the chromosomes in the meristematic nuclei were active,starch grains were accumulated in the meristematic cell mass and the vessels had developed well in xylem.To observe the tissue histochemical changes,PAS staining was carried out on the sections from different parts of the clove at the differentiation stage.The content of sugar granules was rich,the cytoplasm was dense and the staining was dark.The concentration gradient of sugar granules existed between the meristematic cells and their surrounding cells.
作者
刘卓雅
刘秀慧
贾会霞
宋江萍
李锡香
赵丹琦
张晓辉
阳文龙
王海平
LIU Zhuoya;LIU Xiuhui;JIA Huixia;SONG Jiangping;LI Xixiang;ZHAO Danqi;ZHANG Xiaohui;YANG Wenlong;WANG Haiping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation in the Ministry of Agriculturen and Rural Affairs,Beijing 100081,China;Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2021年第7期1409-1417,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31872946)
国家重点研发计划课题项目(2016YFD0100204)
国家现代农业产业技术体系建设专项资金项目(CARS-24-A-01)
中国农业科学院创新工程项目(CAAS-ASTIP-2017-IVFCAAS)
中央级公益性科研院所基本科研业务费专项(IVF-BRF2018001)
农业农村部作物种质资源保护项目
农业农村部作物种质资源安全保存项目
农业农村部园艺作物生物学与种质创制重点实验室项目
国家园艺种质北京蔬菜分库运行服务项目(NHGRC2021-NH0)
国家作物种质资源数据中心观测监测项目(ZX01NK-03)。
关键词
大蒜
鳞芽发育
解剖观察
组织化学分析
garlic
Allium sativum
clove development
anatomical observation
histochemistry analysis
