摘要
目的:探究隐源性卒中(CS)患者肠道炎症及肠道屏障的变化情况及其临床意义。方法:采用ELISA法检测53例CS患者及53例对照者粪便炎症标记物粪钙卫蛋白(FC)、粪乳铁蛋白(FLF)含量,选取8例CS患者及5例对照者的肠镜活检标本行HE染色及免疫组化染色检测紧密连接蛋白Occludin与ZO-1蛋白表达水平。结果:CS组粪便FC、FLF含量高于对照组(P<0.001)。与对照组相比,CS组肠黏膜炎性细胞浸润增加,肠组织ZO-1和Occludin表达水平降低(P<0.001)。结论:CS患者存在肠道炎症及肠道屏障改变,其可能是促成CS发生发展的重要因素。
Aim:To investigate the changes of intestinal inflammation and intestinal barrier in patients with cryptogenic stroke(CS)and its clinical significance.Methods:The fecal inflammation markers fecal calprotectin(FC)and fecal lactoferrin(FLF)in 53 patients with CS and 53 healthy controls were analyzed by ELISA.Intestinal biopsy specimens selected from 8 CS patients and 5 healthy subjects were performed for HE staining and immunohistochemical staining to detect tight junction protein Occludin and ZO-1.Results:The levels of FC and FLF in fecal samples from the CS group were higher than those in the control group(P<0.001).Compared with the control group,the inflammatory cell infiltration of intestinal mucosa in the CS group was increased,and the expression levels of ZO-1 and Occludin was lower(P<0.001).Conclusion:There are intestinal inflammation and intestinal barrier changes in patients with CS,which may be an important factor in the occurrence and development of CS.
作者
项舒雅
崔慧丽
刘晗
夏丹豪
滕军放
王雪晶
XIANG Shuya;CUI Huili;LIU Han;XIA Danhao;TENG Junfang;WANG Xuejing(Department of Neurology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Key-Disciplines Laboratory for Clinical Medicine of Henan Province,Zhengzhou 450052)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2021年第4期497-500,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金面上项目(81873791)。
关键词
隐源性卒中
肠道炎症
肠道屏障
cryptogenic stroke
intestinal inflammation
intestinal barrier
