摘要
目的磁液悬浮心室辅助装置(以下简称为血泵)主动停工在体实验动物研究,旨在观察停泵后血液的反流情况和血泵的血液相容性、生物相容性,以及实验动物生理状态,为后期实验以及临床应用积累可靠数据。方法选用健康绵羊1只,左侧开胸建立左心辅助模型。正常运行6周后主动停泵,停服抗凝药物华法林。观察实验动物生理状态,记录血泵停工羊术前及1-12周血液、生化、凝血检查结果并进行研究。血泵停工后16周进行超声检查。结果左心辅助模型建立成功,血泵运行期间工况良好,实验羊在血泵停工前后6周精神状态良好、活动自如、进食良好、尿便正常。血泵停工后16周的超声显示人工血管未堵塞,有反向血流。结论此血泵停工,对血液相容性、对实验羊生理状态和脏器功能未见明显影响,人工血管通畅且未见血栓,对左心功能无明显影响。为后期临床应用过程中出现意外停泵的处理提供一定参考依据。此血泵血液相容性好。后续将进行左心辅助低剂量抗凝治疗及多组停工实验进行不断完善。
We aimed to characterize the blood reflux,blood compatibility,and biocompatibility of a blood pump after deactivating the pump in an animal in order to facilitate further studies and clinical application.Methods A leftventricular assist device was implanted into a healthy sheep via a left thoracic approach.After 6 weeks of normal operation,the pump was stopped,as was warfarin administration.The physiological status of the animal was then monitored,and blood biochemical and coagulation testing was performed before the procedure and between 1 and 12 weeks after the pump was deactivated.Ultrasonographic examination was performed 16 weeks after the pump was deactivated.Results The left-sided cardiac assistance model was established successfully,and 6 weeks after deactivation of the pump the sheep displayed normal behavior and normal physiology,with no significant changes in the test result.Ultrasonography showed that the artificial blood vessel was not blocked and there was reverse blood flow 16 weeks after the blood pump was stopped.Conclusions Deactivation of a blood pump was not associated with effects on blood compatibility,physiological state,or organ function in a sheep.The artificial blood vessel was not obstructed,no thrombosis was found,and left-sided cardiac function was not significantly affected.Thus,this blood pump has good compatibility,and the study has provided a reference for the clinical treatment of unexpected pump failure.Further studies should assess the impact of low-dose anticoagulant therapy and repeated deactivation on physiology and cardiac function.
作者
王帆
张杰民
刘天文
刘晓程
WANG Fan;ZHANG Jiemin;LIU Tianwen;LIU Xiaocheng(TEDA International Cardiovascular Hospital,Tianjin 300457,China)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2021年第4期482-489,共8页
Acta Laboratorium Animalis Scientia Sinica
基金
国家重点研发计划(2017YFC0111005)。
关键词
植入式磁液悬浮心室辅助装置
功能评价
动物实验
在体实验
ventricular assist device
evaluation of blood pump function
animal experiment
in vivo experiment
