摘要
建立了伊维菌素纳米乳中伊维菌素含量的高效液相色谱检测方法(HPLC)。采用C18色谱柱(4.6×250 mm,5μm),以甲醇-乙腈-水(40∶55∶5)为流动相,流速1.0 mL/min,检测波长254 nm,进样量10μL,柱温25℃。伊维菌素浓度在4.6~92.0μg/mL时,其峰面积与浓度线性关系良好(r 2=0.9996),回收率高,平均回收率为98.25%~98.76%。精密度良好,RSD均小于2%,色谱峰图无杂质干扰,稳定性高,本操作方法简便,分析快速,结果准确,适用于伊维菌素纳米乳剂的含量测定。
A method for the content determination of ivermectin nano-emulsion by HPLC was established.HPLC system consisted of C18 column(4.6×250 mm,5μm),methyl alcohol-acetonitrile-water(40∶55∶5)as mobile phase.The flow rate was 1.0 mL/min.The detection wave length was 254 nm.The injection volume was 10μL and column temperature was room temperature.There was no interference occurred from inpurities.The calibration curve was linear in the range of 4.6~92.0μg/mL(r 2=0.9996).The average recovery was 98.25%~98.76%.The method is rapid,simple and accurate for the content determination of ivermectin nano-emulsion.
作者
苗艳
朱庆贺
陈曦
杨旭东
王爽
王丽坤
陈亮
兰世捷
冯万宇
李丹
史同瑞
MIAO Yan;ZHU Qing-he;CHEN Xi;YANG Xu-dong;WANG Shuang;WANG Li-kun;CHEN Liang;LAN Shi-jie;FENG Wan-yu;LI-Dan;SHI Tong-rui(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang,Qiqihar 161005,China)
出处
《中国兽药杂志》
2021年第8期60-65,共6页
Chinese Journal of Veterinary Drug
基金
黑龙江科研机构创新能力提升专项“兽药新药新品种开发”(YC2016D004)
“农业科技创新跨越工程”专项(HNK2019CX15-10)
“农业科技创新跨越工程”专项(HNK2019CX16)。
