摘要
目的探讨耐辐射奇球菌(Deinococcus radiodurans,DR)活菌及细菌破碎物的急性毒性。方法分别将DR高浓度1×10^(9)/m L(高浓度活菌组)、低浓度1×10^(7)/m L(低浓度活菌组)、细菌破碎物高浓度1×10^(9)/m L(高浓度细菌破碎物组)、低浓度1×10^(7)/m L(低浓度细菌破碎物组)以0.4 m L/10 g灌胃KM小鼠,TGY培养基组灌胃相同体积的胰蛋白胨葡萄糖酵母琼脂培养基(tryptone glucose yeast agar medium),间隔8 h,灌胃2次后,观察14 d,以小鼠行为学、脏器系数、体质量、血常规(白细胞、淋巴细胞、红细胞、血红蛋白、红细胞压积、平均红细胞体积、血小板)以及血浆生化指标(谷丙转氨酶、谷草转氨酶、总蛋白、肌酐、肌酸激酶、镁、磷、肌酸激酶MB型同工酶、乳酸脱氢酶)变化作为评判依据,比较分析耐辐射奇球菌的毒性。结果不同剂量组的小鼠,均未见分泌物、大小便、呼吸及运动异常,灌胃后0.5 h至实验结束,未观察到反应迟钝、瞳孔改变、眼球凸出、眼睑下垂、皮肤颜色改变等异常,受试小鼠体质量、血常规和血液生化指标与TGY培养基组比较,无显著差异,脾脏系数在低浓度细菌破碎物组有显著增大,病理学检查,未见实质性病变。结论DR活菌及其细菌破碎物对小鼠无急性毒性,为DR在临床中的拓展应用提供实验依据。
Objective To investigate the acute toxicity of Deinococcus radiodurans(DR)and its broken products.Method The KM mice were administrated with 0.4 m L/10 g of 1×10^(9)/m L DR(high concentration DR group),1×10^(7)/m L DR(low concentration DR group),1×10^(9)/m L DR broken products(high concentration DR debris group),1×10^(7)/m L DR broken products(low concentration DR debris group)and Tryptone Glucose Yeast agar medium(TGY medium group),respectively.After intragastrically administrated twice a day every 8 hours,the mice were observed for 14 days,then the organ coefficient,body weight,blood routine(white blood cell,lymphocyte,red blood cell,hemoglobin,hematocrit,mean corpuscular volume,platelet)and plasma biochemical indexes(alanine aminotransferase,aspartate aminotransferase,total protein,creatinine,creatine kinase,magnesium,phosphorus,creatine kinase MB isoenzyme,lactate dehydrogenase)were used to analyze and evaluate the toxicity of DR.Result No abnormal secretion,defecation,respiration and movement were found in mice in different dose groups.From 0.5 hour after administration to the end of the experiment,no abnormalities such as slow reaction,pupil change,eyeball protrusion,eyelid ptosis and skin color change were observed in the test mice.There were no significant differences in body weight,blood routine test and blood biochemical indexes in the trial groups compared with TGY group.The spleen coefficient was significantly increased in the low-dose group,and the spleen pathological examination showed that there was no substantial lesion.Conclusion The live and broken DR have no acute toxicity to mice.This experiment provides experimental basis for clinical application of DR.
作者
许琴
宋来阳
张蕾
李佳佳
马娜
江俊峰
刘爱中
蒲凤萍
邓培
刘江伟
王玮
XU Qin;SONG Laiyang;ZHANG Lei;LI Jiajia;MA Na;JIANG Junfeng;LIU Aizhong;PU Fengping;DENF Pei;LIU Jiangwei;WANG Wei(General Hospital of Xinjiang Military Command,Key Laboratory of Special Environmental Medicines of Xinjiang,Urumqi 830000,China;The 948,A Army Hospital of PLA,Wusu 833000,China;Sciences and Technology College,Xinjiang Agricultural University,Urumqi 830091,China;Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,The Key Laboratory of Special Environmental Microorganism of Xinjiang,Urumqi 830091,China)
出处
《实验动物科学》
2021年第3期17-22,共6页
Laboratory Animal Science
基金
全军实验动物专项科研课题(SYDW[2018]10号)。
关键词
耐辐射奇球菌
脏器系数
生化指标
小鼠
急性毒性
Deinococcus radiodurans
organ coefficient
biochemical indexes
mice
acute toxicity
