摘要
目的:研究福建地区经典型Kaposi肉瘤感染的病毒亚型。方法:收集经典型Kaposi肉瘤患者临床资料及石蜡包埋组织标本24例,利用QIAamp® DNA FFPE Tissue试剂盒提取患者皮损蜡块组织中的DNA,设计扩增人类疱疹病毒(HHV)-8(ORF-K1、T0.7/K12、ORF-K14.1/K15、ORF26及ORF75)基因的引物,巢式PCR扩增提取的DNA,然后进行双向测序,再采用SnapGene软件对测序结果进行确认,从而确定HHV-8各病毒亚型的分布情况。结果:24例福建地区经典型Kaposi肉瘤均来源于汉族人,皮损好发于老年人双下肢,以结节为主,患者感染HHV-8不同基因扩增阳性率:ORF26 100.00%、ORF-K133.33%、T0.7/K12 29.17%、ORF-K14.1/K15 33.33%、ORF75 25.00%。序列比对分析显示24例经典型Kaposi肉瘤患者感染HHV-8 ORF26的病毒亚型均为C亚型。结论:福建地区经典型Kaposi肉瘤感染HHV-8的基因型主要为ORF26,且均为C亚型。
Objective:To study the virus subtypes of classic Kaposi sarcoma(KS)in Fujian province.Methods:The clinical data and paraffin-embedded tissue specimens of classic KS were collected and analyzed.Then viral DNA was extract from the patient′s lesion using the QIAamp® DNA FFPE Tissue kit,and different subtypes of HHV-8(ORF-K1,T0.7/K12,ORFK14.1/K15,ORF26 and ORF75)were amplified by nested-PCR.The viral DNA was bidirectional sequenced.The sequences were confirmed by SnapGene software to determine the HHV-8 subtypes.Results:All 24 KS cases in Fujian province were of Han nationality.Most lesions were nodular in the lower limbs of the elderly.The expression rates of ORF26,ORF-K1,T0.7/K12,ORF-K14.1/K15 and ORF75 were 100.00%,33.33%,29.17%,33.33%and 25.00%respectively.The sequence alignment results showed that HHV-8 ORF26 was expressed in subtype C in all cases.Conclusion:The infected virus of classic KS in Fujian province was mainly subtype C of ORF26.
作者
林心瑜
彭佳美
陈丽芬
方芳
赵雁
纪超
程波
纪明开
LIN Xin-yu;PENG Jia-mei;CHEN Li-fen;FANG Fang;ZHAO Yan;JI Chao;CHENG Bo;JI Ming-kai(Department of Dermatology,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2021年第8期460-466,共7页
Journal of Clinical Dermatology
基金
福建省科技厅引导性项目(2018Y0027)资助项目。
