摘要
目的探讨大株红景天胶囊(LPRC)提高小鼠耐常压缺氧能力和抗疲劳作用及其机制。方法6~8周龄雄性BALB/c小鼠按体重和自主活动性均衡随机分为对照组和LPRC 0.35,0.70和1.40 g·kg^(-1)组,每组10只。ig给药7 d后,采用低氧混合气体缺氧和密闭缺氧模型观察LPRC耐缺氧作用;采用负重游泳实验和转棒实验观察LPRC抗疲劳作用;采用比色法检测小鼠非负重游泳力竭后肝和腓肠肌组织中糖原含量,以及血清中疲劳相关指标[尿素氮(BUN)和乳酸(LA)含量及乳酸脱氢酶(LDH)活性]和氧化应激相关指标[超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量]。结果与对照组相比,LPRC 0.70和1.40 g·kg^(-1)组小鼠低氧混合气体缺氧存活时间和负重游泳时间显著延长(P<0.05,P<0.01);LPRC 1.40 g·kg^(-1)组小鼠转棒实验学习期和测试期在棒时间显著延长(P<0.05,P<0.01);LPRC组小鼠非负重游泳力竭后肝和腓肠肌组织中糖原含量及血清中LDH,SOD和GSH-Px活性显著升高(P<0.05,P<0.01),而血清中BUN,LA和MDA含量显著降低(P<0.05,P<0.01)。结论LPRC可显著提高小鼠耐常压缺氧和抗疲劳能力,其作用可能与增加糖原储备、清除代谢物堆积及增强抗氧化活性有关。
OBJECTIVE To explore the effect of large plant Rhodiola capsules(LPRCs)on normobaric hypoxia tolerance and anti-fatigue action in mice.METHODS Male BALB/c mice aged 6 to 8 weeks were randomly divided into control,LPRC 0.35,0.70,and 1.40 g·kg^(-1) groups(n=10).Mice were ig administered with saline or LPRC for 7 d,respectively.Anoxia endurance of LPRCs was assessed by a gas mixture of low oxygen test and hypoxia in hermetic space test.The anti-fatigue action of LPRCs was evaluated by a weight-loaded swimming test and rotarod test.The colorimetric method was used to detect the changes of glycogen contents in the liver and gastrocnemius tissue,fatiguerelated indexes[including the content of blood urea nitrogen(BUN)and lactic acid(LA),the activity of lactate dehydrogenase(LDH)]and oxidative stress-related indexes[including the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),the content of malondialdehyde(MDA)]in serum in exhaustive mice after non-load swimming.RESULTS The hypoxia survival time and weight-loaded swimming time of mice in LPRC 0.70 and 1.40 g·kg^(-1) groups were significantly longer than those of the control group(P<0.05,P<0.01).The time in rotarod of learning phase and test phase of mice in LPRC1.40 g·kg^(-1) group was significantly longer than that of the control group(P<0.05,P<0.01).After nonload swimming,the contents of liver glycogen and muscle glycogen,and the activities of LDH,SOD,and GSH-Px in the LPRC groups were significantly higher than in the control group(P<0.05,P<0.01).Simultaneously,BUN,LA,and MDA levels in LPRC groups were markedly lower than those of the control group(P<0.05,P<0.01).CONCLUSION LPRCs can improve normobaric hypoxia tolerance and antifatigue action,and this effect may be related to the increase in glycogen reserves,clearing of metabolite accumulation and enhancement of antioxidant activity.
作者
王艺博
王峰
肖智勇
周文霞
张永祥
WANG Yi-bo;WANG Feng;XIAO Zhi-yong;ZHOU Wen-xia;ZHANG Yong-xiang(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of Toxicology and Medical Countermeasure,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)
出处
《中国药理学与毒理学杂志》
CAS
北大核心
2021年第6期420-426,共7页
Chinese Journal of Pharmacology and Toxicology
关键词
大株红景天
缺氧
疲劳
氧化应激
large plant Rhodiola capsule
hypoxia
fatigue
oxidative stress
