摘要
目的应用生物信息学预测分析Hrp1蛋白结构功能和生物学特性。方法生物信息学软件ORF Finder、SOSUI、Protpapram、ProtScale、Signal IP、Tmpred、TMHMM、NetNGlyc、NetPhos-3.1-Servera、PSORT、WoLF PSORT、TargetP-2.0-CBS、NLStradamus、SOMPA、SWISS-MODEL、IEDB、SYFPEITHI、RANKPEP、BLAST、DNAStar、STRING对Hrp1蛋白的理化性质、结构功能、生物学特性、抗原表位及蛋白相互作用进行预测分析。结果Hrp1蛋白原子总数2166,等电点4.96,不稳定性指数19.62,脂肪族氨基酸指数100.35,平均疏水性0.042,有跨膜区无信号肽,1个糖基化位点6个磷酸化位点,二级结构以α-螺旋为主。其亚细胞定位于细胞质,属于稳定的亲水性蛋白。经预测,Hrp1蛋白含有4个B细胞表位,多个T细胞表位,且其相互作用蛋白分别为Rv2627c、Rv2628、Rv1738、devR、Rv2624c、TB31.7、rip3、pfkB、guaA、hspX,并参与多种生物学过程。结论生物信息学分析Hrp1蛋白为稳定亲水性胞浆蛋白,具有良好的抗原表位,为结核病诊断及治疗的潜在候选因子,是探索结核分枝杆菌持久性感染机制的重要研究靶标,可作为抗结核多肽疫苗的候选蛋白。
We applied bioinformatics to predict,analyze and understand the structure,function,and biological characteristics of Hrp1 protein.Bioinformatics software,including ORF Finder,SOSUI,Protpapram,ProtScale,Signal IP,Tmpred,TMHMM,NetNGlyc,NetPhos-3.1-Servera,PSORT,WoLF PSORT,TargetP-2.0-CBS,NLStradamus,SOMPA,SWISS-MODEL,IEDB,SYFPEITHI,RANKPEP,BLAST,DNAStar and STRING,were used to predict and analyze the physicochemical properties,structure,function,biological characteristics,and epitope and protein interaction of Hrp1 protein.The results showed that Hrp1 protein has a total number of atoms of 2166,an isoelectric point of 4.96,an instability index of 19.62,an aliphatic amino acid index of 100.35 and an average hydrophobicity of 0.042.It additionally has a transmembrane region,no signal peptide,one glycosylation site and six phosphorylation sites.Its secondary structure is dominated byα-helices,and its subcellular localization is in the cytoplasm.It is thus a stable hydrophilic protein.Bioinformatic prediction and analysis indicated that Hrp1 protein contains four B cell epitopes and multiple T cell epitopes;its interacting proteins are Rv2627c,Rv2628,Rv1738,devR,Rv2624c,TB31.7,rip3,pfkB,guaA and hspX;and it participates in a variety of biological processes.Through bioinformatic analysis,this study reveals that Hrp1 is a stable hydrophilic cytoplasmic protein that has good epitopes and may be a potential candidate factor in the diagnosis and treatment of tuberculosis.Therefore,Hrp1 is an important research target in exploring the mechanism of persistent mycobacterium tuberculosis infection and may be used as a candidate protein for the development of an anti-tuberculosis polypeptide vaccine.
作者
刘静
吴芳
张杰
董江涛
柳小玲
梁粟
王菊
张春军
吴江东
章乐
赵海军
张万江
LIU Jing;WU Fang;ZHANG Jie;DONG Jiang-tao;LIU Xiao-ling;LIANG Su;WANG Ju;ZHANG Chun-jun;WU Jiang-dong;ZHANG Le;ZHAO Hai-jun;ZHANG Wan-jiang(Xi'an Jiaotong University City College,Shanxi 710018,China;Department of Pathophysiology,Shihezi University School of Medicine/Xinjiang High Incidence of Local and Ethnic Diseases,Key Laboratory of the Ministry of Education,Shihezi 832002,China;The First Affiliated Hospital of Medical College of Shihezi University,Shihezi 832002,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2021年第8期669-677,共9页
Chinese Journal of Zoonoses
基金
NSFC-新疆联合基金项目(No.U1803127)
国家自然基金项目(No.82060021)
兵团重点领域科技攻关计划项目(No.2018AB019)联合资助。
