摘要
该文研究了南极磷虾油(antarctic krill oil,AKO)对衰老模型小鼠的抗氧化作用。无特定病原体(specific pathogen free,SPF)级癌症研究学会(institute of cancer research,ICR)健康雄性小鼠培养达到8~10月龄构建衰老模型小鼠,将衰老模型小鼠随机分成4组,每组10只,设置南极磷虾油处理低剂量组(L-AKO)0.075 g/kg·BW、中剂量组(M-AKO)0.150 g/kg·BW、高剂量组(H-AKO)0.450 g/kg·BW和阴性对照组,阴性对照组给予等体积的食用植物油,每天灌胃1次,连续灌胃30 d。30 d后,测定过氧化脂质(lipid peroxide,LPO)含量[以丙二醛(malondialdehyde,MDA)含量计]、还原型谷胱甘肽(reduced glutathione,GSH)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力及蛋白质羰基(protein carbonyl,PCO)含量。结果表明,AKO中、高剂量组能分别显著和极显著提高血清SOD活力(P<0.05,P<0.01)和GSH含量(P<0.05,P<0.01),显著降低血液中LPO含量(P<0.05),PCO含量无显著变化(P>0.05)。研究证明AKO对衰老模型小鼠具有抗氧化作用。
The aim of this study was to explore the antioxidant effect of Antarctic krill oil(AKO)on aging model mice.SPF-ICR healthy male mice aged 8-10 months were randomly divided into 4 groups,low-dose group,medium-dose group,high-dose krill oil group and one negative control group.Ten mice in each group and were given a dose of 0.075 g/kg·BW,0.150 g/kg·BW,0.450 g/kg·BW and corn germ oil respectively.The four mice groups were dosed by gavage once a day for 30 days.After 30 days,the contents of lipid peroxide(LPO)(calculated by the content of MDA),reduced glutathione(GSH),superoxide dismutase(SOD)and protein carbonyl(PCO)were determined.The results showed that compared with the control group,the activity of serum SOD and serum level of GSH increased considerably(P<0.05)in the medium dose of the krill oil group,and even significantly(P<0.01)in the high-dose group.The level of LPO in the medium dose group was lower(P<0.05)than the control group.However,there was no statistical difference for the level of PCO between the medium dose group and the control group(P>0.05).These results revealed AKO has an antioxidant effect on aging model mice.
作者
李辉
王廉馨
韩昀稣
林丹妮
曲丽娜
LI Hui;WANG Lianxin;HAN Yunsu;LIN Danni;QU Lina(School of Life Sciences,Liaoning University,Shenyang 110036,China;Dalian Municipal Central Hospital Affiliated of Dalian Medical University,Dalian 116033,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第16期123-127,共5页
Food and Fermentation Industries
关键词
南极磷虾油
衰老模型小鼠
抗氧化作用
虾青素
磷脂
Antarctic krill oil
aging model mice
antioxidation
astaxanthin
phospholipids
