摘要
目的拟通过建立MCT诱导的PAH大鼠模型,观察人脐带间充质干细胞(UC-MSCs)干预对大鼠PAH和右心衰竭的治疗效应。方法实验动物采用完全随机设计方法分为3组(空白对照组、PAH组和UC-MSCs组),在MCT腹腔注射1周后进行干预,UC-MSCs组舌下静脉注射UC-MSCs悬液,空白对照组和PAH组舌下静脉注射等量生理盐水。第4周大鼠行右心超声心动图、右心导管测压、右心肥厚指数以及肺组织病理等检测。采用SPSS 23.0统计软件进行统计分析,多组间均数比较采用单因素方差分析,P<0.05为差异有统计学意义。结果与对照组相比,PAH组大鼠右心室游离壁厚度(RVWT)和右心室内径(RVID)显著增大[(1.56±0.14)mm比(0.71±0.09)mm,(4.59±0.21)mm比(1.92±0.18)mm,P<0.05],肺动脉血流加速时间与射血时间比值(PAT/PET)显著升高[(39.4±2.68)%比(20.49±2.69)%,P<0.05],右心收缩压(RVSP)和右心肥厚指数(RVHI)显著增高[(52.13±3.38)mm Hg比(14.94±0.75)mm Hg,(68.28±7.25)%比(28.63±1.76)%,P<0.05],肺小动脉血管壁厚度(WT)明显增厚[(24.15±2.54)%比(10.15±0.62)%,P<0.05]。与PAH组相比,UC-MSCs组RVWT和RVID显著减小[(1.17±0.05)mm比(1.56±0.14)mm,(2.95±0.4)mm比(4.59±0.21)mm,P<0.05],PAT/PET明显下降[(25.21±1.31)%比(39.4±2.68)%,P<0.05],RVSP和RVHI明显降低[(38.55±1.98)mm Hg比(52.13±3.38)mm Hg,(44.29±3.98)%比(68.28±7.25)%,P<0.05],WT明显变薄[(15.82±1.75)%比(24.15±2.54)%,P<0.05]。结论利用MCT腹腔注射成功制备PAH大鼠模型,经舌下静脉注射UC-MSCs可以显著降低肺动脉压力,改善右心功能,逆转肺血管重构。
Objective To observe the therapeutic effect of umbilical cord mesenchymal stem cells(UCMSCs)on pulmonary arterial hypertension(PAH)and right heart failure in a Monocrotaline(MCT)induced PAH rat model.Methods The experimental animals were divided into 3 groups by completely random design(control group,PAH group and UC-MSCs group).After 1 week of intraperitoneal injection of MCT,the UC-MSCs group was injected with UC-MSCs suspension intravenously,while the control group and PAH group were injected with the same amount of normal saline intravenously.At the 4th week,the rats were examined by right ventricular echocardiography,right ventricular catheterization manometry,right cardiac hypertrophy index and lung pathology.SPSS 23.0 statistical software was used for statistical analysis.One-way analysis of variance was used to compare the mean between groups.P<0.05 indicates that the difference was statistically significant.Results Compared with the control group,right ventricular free wall thickness(RVWT)and right ventricular inner diameter(RVID)in PAH group were significantly increased[(1.56±0.14)mm vs.(0.71±0.09)mm,(4.59±0.21)mm vs.(1.92±0.18)mm,P<0.05].The ratio of pulmonary acceleration time to ejection time(PAT/PET)was significantly increased[(39.4±2.68)%vs.(20.49±2.69)%,P<0.05].Right ventricular systolic pressure(RVSP)and right ventricular hypertrophy index(RVHI)were significantly increased[(52.13±3.38)mm Hg vs.(14.94±0.75)mm Hg,(68.28±7.25)%vs.(28.63±1.76)%,P<0.05].The arterial wall thickness(WT)was significantly thickened[(24.15±2.54)%vs.(10.15±0.62)%,P<0.05].Compared with PAH group,RVWT and RVID in UC-MSCs group were significantly decreased[(1.17±0.05)mm vs.(1.56±0.14)mm,(2.95±0.4)mm vs.(4.59±0.21)mm,P<0.05].PAT/PET were significantly decreased[(25.21±1.31)%vs.(39.4±2.68)%,P<0.05].RVSP and RVHI were significantly decreased[(38.55±1.98)mm Hg vs.(52.13±3.38)mm Hg,(44.29±3.98)%vs.(68.28±7.25)%,P<0.05].WT was significantly thinner[(15.82±1.75)%vs.(24.15±2.54)%,P<0.05].Conclusion UC-MSCs coul
作者
张玉海
李力兵
王亮
吴远斌
毛磊
李钦
王嵘
ZHANG Yu-hai;LI Li-bing;WANG Liang;WU Yuan-bin;MAO Lei;LI Qin;WANG Rong(Department of Thoracic Surgery,Baotou Clinical Medical College,Inner Mongolia Medical University,Baotou 014040y China;Department of Cardiovascular Surgery,Chinese PLA General Hospital,Beijing 100853,China)
出处
《中国心血管病研究》
CAS
2021年第8期757-763,共7页
Chinese Journal of Cardiovascular Research
关键词
肺动脉高压
右心衰竭
野百合碱
脐带间充质干细胞
Pulmonary arterial hypertension
Right heart failure
Monocrotaline
Umbilical cord mesenchymal stem cells
