摘要
小鼠杂交瘤单克隆抗体来源稳定、后期易制备、产量高,是免疫学中使用最为普遍的抗体。传统的耗时费力的杂交瘤制备技术无法满足日益增长的市场需求。文中从抗原设计筛选、B细胞富集与筛选、骨髓瘤细胞的改造、融合技术的改进、阳性杂交瘤细胞筛选及单克隆抗体性能快速测定中所涉及的快速制备技术方面进行阐述,以期为系统化的小鼠杂交瘤单克隆抗体的快速制备方法提供参考。
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
作者
方水琴
刘程
马俊飞
闫帅帅
许东坡
刘箐
Shuiqin Fang;Cheng Liu;Junfei Ma;Shuaishuai Yan;Dongpo Xu;Qing Liu(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2021年第7期2293-2306,共14页
Chinese Journal of Biotechnology
基金
国家重点研发计划(Nos.2018YFC1602900,2018YFC1602500)资助。
关键词
小鼠杂交瘤
单克隆抗体
快速制备
抗原特异性
mouse hybridoma
monoclonal antibody
rapid preparation
antigen-specific
