摘要
目的:探讨白细胞介素-23(IL-23)和辅助性T细胞17(Th17)在布鲁杆菌性脊柱炎(BS)中的作用。方法:选取2015年1月至2020年4月河北北方学院附属第一医院收治的111例BS患者为研究对象,其中急性期患者61例(急性期组),慢性期患者50例(慢性期组),同期选取医院健康体检对象120例为对照组;对BS患者治疗前血清试管凝集试验(SAT)滴度、C反应蛋白(CRP)、红细胞沉降率(ESR)进行检测;酶联免疫吸附(ELISA)法检测受试者血清IL-23、IL-17、肿瘤坏死因子-α(TNF-α)、核因子-κB受体活化因子配体(RANKL)、基质金属蛋白酶-2(MMP-2)水平;流式细胞术检测受试者Th17细胞水平;Pearson法分析BS患者血清IL-23与Th17细胞水平及血清IL-23、Th17细胞与RANKL、MMP-2之间的相关性。结果:急性期组SAT滴度≥1∶100、CRP≥5mg/L、ESR≥20mm/h患者比例均高于慢性期组(P<0.05);与对照组比较,慢性期组、急性期组Th17细胞、血清TNF-α、IL-17、IL-23、RANKL、MMP-2水平均升高(P<0.05);与慢性期组比较,急性期组Th17细胞、血清TNF-α、IL-17、IL-23、RANKL、MMP-2水平均升高(P<0.05);BS患者血清IL-23与Th17细胞、RANKL、MMP-2水平呈正相关(P<0.05),Th17细胞与RANKL、MMP-2水平呈正相关(P<0.05)。结论:IL-23、Th17可能参与BS的发病机制,本研究可能为BS发病研究提供临床依据。
Objective:To investigate the roles of interleukin-23(IL-23)and T helper cell 17(Th17)in brucellosis spondylitis(BS).Methods:A total of 111 patients with BS admitted to the First Hospital Affiliated to Hebei North University from January 2015 to April 2020 were selected as the research objects,including 61 patients in acute phase(acute phase group)and 50 patients in chronic phase(chronic phase group),at the same time,120 healthy controls were selected as the control group;the serum tube agglutination test(SAT)titer,C-reactive protein(CRP),erythrocyte sedimentation rate(ESR)were detected in patients with BS before treatment;the levels of serum L-23,IL-17,tumor necrosis factor-α(TNF-α),receptor activator of NF-κB ligand(RANKL)and matrix metalloproteinase-2(MMP-2)were detected by enzyme linked immunosorbent assay(ELISA);Th17 cells level were detected by flow cytometry;Pearson method was used to analyze the correlation between serum IL-23 and Th17,between serum IL-23,Th17 and RANKL,MMP-2 in patients with BS.Results:The proportion of patients with SAT titer≥1∶100,CRP≥5mg/L and ESR≥20mm/h in acute phase group was higher than that in chronic phase group(P<0.05);compared with those in the control group,the Th17 cells,expression levels of serum TNF-α,IL-17,IL-23,RANKL and MMP-2 were higher in chronic phase group and acute phase group(P<0.05);compared with those in chronic phase group,the Th17 cells,expression levels of serum TNF-α,IL-17,IL-23,RANKL and MMP-2 in acute phase group were significantly higher(P<0.05);the level of IL-23 in BS patients was positively correlated with Th17 cells,RANKL and MMP-2(P<0.05),and Th17 cells was positively correlated with RANKL and MMP-2(P<0.05).Conclusion:IL-23 and Th17 may be involved in the pathogenesis of BS.This study may provide clinical basis for the study of BS.
作者
贾永利
左宪宏
杨新明
张培楠
孟宪勇
胡长波
JIA Yongli(The First Hospital Affiliated to Hebei North University, Hebei Zhangjiakou 075000, China)
出处
《河北医学》
CAS
2021年第8期1251-1255,共5页
Hebei Medicine
基金
河北省2019年度医学科学研究课题计划项目(编号:20190120)
2018年度河北省卫生健康技术研究暨成果转化重点项目(编号:zh2018014)
2018年度河北省医学适用技术跟踪项目
(编号:G2018074)。
