摘要
利用漆酶/TEMPO体系对壳寡糖(COS)分子进行选择性氧化,制备6-羧基壳寡糖(C-COS).通过傅里叶红外光谱(FTIR)、核磁共振波谱(13C NMR)及酸碱电导滴定对样品进行表征分析,并对6-羧基壳寡糖(C-COS)清除活性氧(ROS)能力进行探究.结果表明:漆酶/TEMPO体系成功将壳寡糖氧化为6-羧基壳寡糖;6-羧基壳寡糖清除活性氧的能力强于壳寡糖且随溶液浓度增加而逐渐提高.C-COS对羟基自由基、过氧化氢、烷氧自由基及超氧阴离子自由基的最大清除率分别达98%、99%、59%、39%.因此,6-羧基壳寡糖在医药及化妆行业有良好应用前景.
In this study,6-carboxyl chitosan oligosaccharide(C-COS)was prepared by selective oxidation of laccase/TEMPO.The samples were characterized and analyzed by Fourier infrared spectroscopy(FTIR),nuclear magnetic reso-nance(13C NMR)and acid-base conductance titration,and the scavenging ability of 6-carboxyl chitosan oligosaccharide(C-COS)on reactive oxygen species(ROS)was investigated.The result showed that laccase/TEMPO system successfully oxi-dized chitosan oligosaccharides to 6-carboxyl chitosan oligosaccharides;the scavenging ability of 6-carboxyl chitosan oligo-saccharides was higher than that of chitosan oligosaccharides and the scavenging ability was gradually increased with theincrease of sample solution concentration.The maximum scavenging capacity of C-COS on hydroxyl radical,hydrogen per-oxide,alkoxide radical and superoxide anion radical was 98%,99%,59%and 39%,respectively.Therefore,6-carboxyl chitosan oligosaccharide have good application prospect in medicine and cosmetics industry.
作者
杨晓莉
唐延东
张方东
裴继诚
YANG Xiaoli;TANG Yandong;ZHANG Fangdong;PEI Jicheng(Tianjin Key Laboratory of Pulp and Paper,College of Light Industry Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
2021年第4期73-80,共8页
Journal of Tianjin University of Science & Technology
关键词
6-羧基壳寡糖
ROS清除
作用机理
6-carboxyl chitosan oligosaccharide
ROS removal
mechanism of action
