摘要
目的观察镰形棘豆总黄酮(FOFB)对特发性肺纤维化(IPF)模型大鼠α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(COLⅠ)和Ⅲ型胶原蛋白(COLⅢ)表达的影响。方法借助喉镜行气管插管和滴注博来霉素(5 mg·kg^(-1))建立IPF大鼠模型。按照体重将SD大鼠随机分为6组:空白组、模型组、低中高3个剂量实验组(100,200,400 mg·kg^(-1)镰形棘豆总黄酮)和阳性对照组(20 mg·kg^(-1)醋酸泼尼松),每组10只。造模后第15天,开始灌胃给药,1次/天,连续4周。以Masson染色法评价肺纤维化程度,以免疫组化法检测肺组织中α-SMA、COLⅠ和COLⅢ蛋白的表达(组织化学评分)。结果空白组、模型组、低中高3个剂量实验组和阳性对照组的肺纤维化评分分别为(0.00±0.00),(3.16±0.41),(2.82±0.41),(2.33±0.52),(0.50±0.54)和(0.33±0.52)分;这6组的α-SMA蛋白表达水平分别为(68.34±3.88),(127.94±1.66),(112.43±16.28),(95.88±3.71),(95.12±3.58)和(75.86±6.05)分;这6组的COLⅠ蛋白表达水平分别为(33.84±0.72),(52.48±5.44),(47.82±5.31),(43.06±1.66),(42.23±2.02)和(39.56±3.96)分;这6组的COLⅢ蛋白表达水平分别为(54.74±23.52),(124.00±2.90),(105.27±13.70),(97.34±9.13),(92.88±4.68)和(76.48±19.73)分。基因结果的趋势与蛋白一致。上述指标:模型组与空白组相比,差异均有统计学意义(均P<0.05);中、高2个剂量实验组和阳性对照组与模型组比较,差异均有统计学意义(均P<0.05)。结论FOFB可能通过下调α-SMA、COLⅠ、COLⅢ的表达而延缓博来霉素诱导的IPF大鼠的纤维化进展。
Objective To observe the effect and mechanism of total flavonoids from Oxytropis falcata Bunge(FOFB)onα-smooth muscle actin(α-SMA),collagenⅠ(COLⅠ)and collagenⅢ(COLⅢ)in idiopathic pulmonary fibrosis(IPF)model rats.Methods IPF model rats were established by endotracheal intubation with laryngoscope and bleomycin(5 mg·kg^(-1)).SD rats were randomly divided into 6 groups according to weight:blank group model group,experimental-L,experimental-M,experimental-H groups(100,200 and 400 mg·kg^(-1) FOFB),positive control group(20 mg·kg^(-1) prednisone acetate),each group included 10 animals.On the 15 th day after modeling,the rats were given intragastric administration once a day for 4 weeks.The degree of pulmonary fibrosis was evaluated by Masson staining.The expression(histochemistry score)ofα-SMA,COLⅠand COLⅢproteins in lung tissue were determined by immunohistochemistry.Results The pulmonary fibrosis score in blank group,model group,experimental-L,experimental-M,experimental-H groups and positive control group were(0.00±0.00),(3.16±0.41),(2.82±0.41),(2.33±0.52),(0.50±0.54),(0.33±0.52)point,respectively;the protein expression level ofα-SMA in the six groups were(68.34±3.88),(127.94±1.66),(112.43±16.28),(95.88±3.71),(95.12±3.58)and(75.86±6.05)point,respectively;the protein expression level of COLⅠin the six groups were(33.84±0.72),(52.48±5.44),(47.82±5.31),(43.06±1.66),(42.23±2.02)and(39.56±3.96)point,respectively;the protein expression level of COLⅢin the six groups were(54.74±23.52),(124.00±2.90),(105.27±13.70),(97.34±9.13),(92.88±4.68)and(76.48±19.73)point,respectively.The trend of gene results was consistent with that of protein.Significant difference of the factors were found between blank group and model group(all P<0.05);There had statistical significance comparison between experimental-M group,experimental-H group,positive control group and model group(all P<0.05).Conclusion FOFB may delay bleomycin-induced fibrosis in IPF rats by down-regulating the expression ofα-S
作者
李欣泽
王彦君
李杨
梁乾坤
陈彦文
杨玲玲
明海霞
LI Xin-ze;WANG Yan-jun;LI Yang;LIANG Qian-kun;CHEN Yan-wen;YANG Ling-ling;MING Hai-xia(Basic Medical College,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Key Laboratory of Molecular Medicine and Prevention and Treatment of Major Diseases with Traditional Chinese Medicine in Universities of Gansu Province,Lanzhou 730000,Gansu Province,China;Institute of Integrative Medicine with Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2021年第15期1999-2002,共4页
The Chinese Journal of Clinical Pharmacology
