摘要
目的:探讨宿主基因HLA-A24、CW1表达、HBV-DNA荧光定量及生化指标ALT、AST变化与慢性乙型肝炎中医临床各证型的相关性。方法:收集符合标准的98例乙型肝炎患者按照中医辨证分型分为肝胆湿热、肝郁脾虚、肝肾阴虚、瘀血阻络及脾肾阳虚5种证型,设40名健康人为正常对照组。实时荧光定量PCR检测血清中的乙肝病毒定量(HBV-DNA)水平及白细胞中的HLA-A24和CW1基因水平,全自动生化分析仪上检测血清中丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST),并分析相关因素与不同中医证型的相关性。结果:HBV-DNA定量检测结果:四组证型之间差异无统计学意义(P>0.05);HLA-A24定量结果:以脾肾阳虚证型最低,四组证型与正常对照组相比较,结果差异有统计学意义(P<0.05);HLA-CW1定量结果以肝胆湿热最低,与肝郁脾虚及脾肾阳虚组之间差异有统计学意义(P<0.05);ALT定量结果以脾肾阳虚证最高,与其它三组(肝郁脾虚、肝肾阴虚及瘀血阻络)结果相比较有统计学意义(P<0.05);AST定量结果以脾肾阳虚证最高,与其它三组(肝胆湿热、肝肾阴虚及瘀血阻络)结果比较差异有统计学意义P<0.05)。相关性比较:HBV-DNA定量与ALT及AST呈正相关(P<0.05);HLA-CW1与HLA-A24呈正相关。结论:不同中医证型乙型肝炎患者结局不同,通过HBV-DNA、肝酶及HLA-A24及HLA-CW1的联合检测可以为临床慢性乙型肝炎中医证型分型提供一个方向性指导。
Objective:To investigate the relationship between the expression of host genes HLA-A24,CW1,HBVDNA fluorescence quantification,and changes in biochemical indicators ALT and AST with various clinical syndromes of chronic hepatitis B.Methods:Collect 98 patients with hepatitis B that meet the criteria and divide them into 5 syndrome types according to TCM syndrome differentiation,liver and gallbladder damp-heat,liver stagnation and spleen deficiency,liver and kidney yin deficiency,blood stasis blocking collaterals,and spleen and kidney yang deficiency.Normal control group.Real-time fluorescent quantitative PCR detects the level of hepatitis B virus(HBV-DNA)in serum and the levels of HLA-A24 and CW1 genes in white blood cells,and detects serum alanine aminotransferase(ALT)and asparagus on an automatic biochemical analyzer Amino acid aminotransferase(AST),and analyze the correlation between related factors and different TCM syndrome types.Results:HBV-DNA quantitative detection results:there was no statistically significant difference between the four groups of syndrome types(P>0.05);HLA-A24 quantitative results:the spleen-kidney-yang deficiency syndrome was the lowest,and the four groups were similar to the normal control group.Compared with the results,the difference was statistically significant(P<0.05);the quantitative results of HLA-CW1 were the lowest in liver and gallbladder damp-heat,which was significantly different from the liver depression and spleen deficiency and spleen and kidney yang deficiency groups(P<0.05);ALT quantitative results The spleen and kidney yang deficiency syndrome is the highest,which is statistically significant compared with the results of the other three groups(liver stagnation and spleen deficiency,liver and kidney yin deficiency,and blood stasis obstruction)(P<0.05);AST quantitative results are the spleen and kidney yang deficiency syndrome the highest,Compared with the other three groups(liver and gallbladder damp-heat,liver and kidney yin deficiency and blood stasis obstructing the
作者
杨小蓉
蔡祥胜
卢汉威
黄演婷
陈小明
YANG Xiaorong;CAI Xiangsheng;LU Hanwei;HUANG Yanting;CHEN Xiaoming(The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangdong Guangzhou 510080,China;Center for Medical Experiments,University of Chinese Academy of Science Shenzhen Hospital,Guangdong Shenzhen 518000,China)
出处
《中医药临床杂志》
2021年第7期1317-1321,共5页
Clinical Journal of Traditional Chinese Medicine
基金
广州市科技计划项目(No.201803040014)。
